盐酸戊乙奎醚对大鼠急性坏死性胰腺炎肺损伤MIF和AQP-5表达的影响

Treatment with penehyclidine hydrochloride reduces the levels of MIF in serum and AQP-5 in bronchoalveolar lavage fluid in rats with acute necrotizing pancreatitis and acute lung injury

目的:探讨盐酸戊乙奎醚对大鼠急性坏死性胰腺炎(acute necrotic pancreatitis,ANP)并发急性肺损伤(acute lung injury,ALI)血清巨噬细胞移动抑制因子(macrophage migration inhibitory factor,MIF)和肺泡灌洗液水通道蛋白-5(aquaporin-5,AQP-5)表达的影响.方法:采用经胰胆管内逆行注射4%牛磺胆酸钠构建大鼠ANP并发ALI模型,100只SD大鼠以是否接受盐酸戊乙奎醚(penehyclidine hydrochloride,PHCD)腹腔注射干预治疗随机分为治疗组和非治疗组,每组再以6h、12h、24h、36h、48h五个取材时间点继续随机分组,共10组,每组10只动物.采用ELISA法测定血清中MIF的浓度和肺泡灌洗液中AQP-5的浓度,同时进行胰腺和肺组织病理形态学观察,肺组织湿/干重比检测,动脉血气分析.结果:相同时间点PHCD治疗组与非治疗组比较显示,血清MIF浓度降低(χ2=20.52,P=0.042),肺泡灌洗液AQ P-5浓度增高(χ2=17.22,P=0.044),胰腺和肺组织病理学损伤减轻,肺组织湿/干质量比值减小(χ2=21.04,P=0.000),动脉血PaO2上升(χ2=503.48,P=0.000)、PaCO2下降(χ2=82.83,P=0.000).结论:PHCD的适当干预对大鼠ANP并发ALI具有一定的治疗效果.

AIM：To investigate the influence of treatment with penehyclidine hydrochloride （PHCD） on the levels of macrophage migration inhibitor factor （MIF） in serum and aquaporin-5 （AQP-5） in bronchoalveolar lavage fluid （BALF） in rats with acute necrotizing pancreatitis （ANP） and acute lung injury （ALI）.METHODS：ANP and ALI was induced in Sprague-Dawley rats by retrograde injection of 4% sodium taurocholate into the biliopancreatic duct.One hundred rats were randomly and equally divided into treatment group and nontreatment group.The treatment group received intraperitoneal injection of PHCD.Each group was further equally and randomly divided into five subgroups for testing at 6 h,12 h,24 h,36 h,and 48 h.The concentrations of MIF in serum and AQP-5 in BALF were determined by enzyme-linked immunosorbent assay （ELISA）.Pancreatic and pulmonary pathomorphology was recorded and compared between the two groups.The wet-to-dry weight ratio of the lung was measured and blood gas analysis was performed.RESULTS：Compared to the non-treatment group,the treatment group had significantly decreased serum levels of MIF （χ 2=20.52,P=0.042）,the wet-to-dry weight ratio of the lung （χ 2=21.04,P=0.000） and arterial PaCO 2 （χ 2=82.83,P=0.000）,significantly increased BALF levels of AQP-5 （χ 2=17.22,P=0.044） and arterial PaO 2 （χ 2=503.48,P=0.000）,and significantly mitigated pancreatic and lung histopathological changes.CONCLUSION：PHCD intervention has an appreciable therapeutic effect on ANP and ALI in rats.