摘要
背景与目的:Ets转录因子家族成员多瘤病毒增强子激活因子3基因(polyomavirus enhanceractivator 3,PEA3)与肿瘤的侵袭和转移密切相关,其在乳腺癌发生、发展中的作用有待探讨。本研究旨在探讨PEA3蛋白在乳腺癌组织中的表达及其与Her-2/neu和乳腺癌临床病理因素的关系。方法:采用免疫组化法检测80例乳腺癌组织中PEA3及Her-2/neu的表达,对Her-2/neu(++)的病例采用显色原位杂交(coloration insitu hybridization,CISH)检测Her-2基因扩增情况。结果:80例乳腺癌组织中PEA3的阳性率57.5%(46/80),高于正常乳腺组织及乳腺纤维腺瘤,与肿瘤大小、组织学分级及腋淋巴结转移均有一定的相关性,且与Her-2/neu的表达呈正相关。PEA3蛋白的阳性表达与乳腺癌患者5年生存率呈负相关。结论:PFA3与乳腺癌浸润转移及预后密切相关,有望作为乳腺癌预后的一个参考指标,其作用机制可能与Her-2/neu相关。
Background and purpose：The role of the polyomavirus enhancer activator 3（PEA3）,a member of subfamily of Ets transcription factors,in breast neoplasia is controversial.This study investigated the relationship between PEA3 expression and Her-2/neu and clinicopathological parameters in breast cancer.Methods： The expressions of PEA3 and Her-2/neu were detected by immunohistochemistry and coloration in situ hybridization （CISH）.Results：The positive rate of PEA3 in breast cancer tissue was 57.5%（46/80）,which was higher than that in tissue of normal breast and fibroadenoma of breast.Upregulation of PEA3 expression in tissue of breast cancer was associated with tumor size,grade,axillary lymph node metastasis and Her-2/neu expression.PEA3 positive expression was negatively correlated with 5-year survival rate of patients with breast cancer.Conclusion：PEA3 may be an important marker in evaluating the invasion,metastasis and prognosis of breast cancer,which involves Her-2 mediated oncogenesis.
出处
《中国癌症杂志》
CAS
CSCD
北大核心
2011年第10期766-770,共5页
China Oncology
基金
上海市卫生局科研计划课题资助项目(N0:2007054)
