抗性淀粉干预对糖尿病大鼠子代糖代谢的影响

Effects of dietary resistant starch on glucose metabolism in offsprings of diabetic rats

目的探讨抗性淀粉干预糖尿病大鼠对其子代糖代谢的影响。方法 20只6周龄雌性2型糖尿病Goto-Kakizaki(GK)大鼠随机分为抗性淀粉组(GK-RS组,n=10)和等能量密度饲料对照组(GK-EC组,n=10),以喂食等能量密度饲料的同龄Wistar雌鼠作为空白对照组(Wistar-EC组,n=10),分组喂养直至分娩。在各组幼鼠中分别随机选取10只雄性幼鼠,常规饲料喂养至8周龄,每周3次记录体质量和摄食量,免疫组织化学法检测胰腺相对β细胞密度,ELISA法测定空腹血清胰岛素水平,口服葡萄糖耐量试验检测空腹血糖和餐后2 h血糖,以稳态模型的胰岛素抵抗指数(HOMA-IR)评估胰岛素敏感性。结果与GK-EC组比较,GK-RS组空腹血糖浓度显著降低(P<0.05),胰腺胰岛素含量明显升高(P<0.05),胰腺相对β细胞密度呈现增高趋势(P=0.07)。GK-EC与GK-RS组幼鼠的摄食量、体质量、HOMA-IR、餐后2 h血糖和血清空腹胰岛素水平比较,差异均无统计学意义(P>0.05)。结论抗性淀粉干预糖尿病大鼠有益于改善其子代发生的糖代谢紊乱。

Objective To investigate the effects of dietary resistant starch on glucose metabolism in offsprings of diabetic rats. MethodsTwenty female Goto-Kakizaki（GK） rats with type 2 diabetes aged 6 weeks were randomized into resistant starch diet group（GK-RS group,n=10） and energy control diet group（GK-EC group,n=10）,and another 10 age-matched female Wistar rats fed with energy control diet were served as blank control group（Wistar-EC group）.Ten male offsprings were randomly selected in each group,and were raised till 8 weeks old on regular chow diet.Body weight and volume of food intake were recorded three time a week,pancreatic relative β cell density was measured by immunohistochemistry,fasting serum insulin concentration was detected by ELISA,concentrations of fasting blood glucose and 2 h postprandial blood glucose were determined by oral glucose tolerance test,and insulin sensitivity was evaluated by homeostasis model assessment of insulin resistance（HOMA-IR）. ResultsCompared with GK-EC group,the concentration of fasting blood glucose significantly decreased（P0.05）,pancreatic insulin content significantly increased（P0.05）,and pancreatic relative β cell density tended to increase in GK-RS group（P=0.07）.There was no significant difference in body weight,volume of food intake,HOMA-IR and concentrations of 2 h postprandial blood glucose and fasting serum insulin between GK-EC group and GK-RS group（P0.05） ConclusionDietary resistant starch has potential in improving glucose metabolism disorder in offsprings born to diabetic dams.