中链脂肪酸辛酸和癸酸对糖尿病小鼠糖代谢的影响

Effect of medium-chain caprylic and capric acids on glucose metabolism in mice with diabetes mellitus

目的观察中链脂肪酸辛酸和癸酸对kkay糖尿病小鼠糖代谢的影响。方法选择4-5周龄雄性kkay小鼠36只,随机分为高脂饲料添加辛酸组(C8),高脂饲料添加中癸酸组(C10)及高脂饲料添加油酸组(0A),每组12只,喂饲12周。观察各组小鼠体重、饲料消耗量、食物功效比、血糖的差异。结果喂养12周后,与油酸组相比,辛酸和癸酸组kkay小鼠体重、体重增加值以及体脂肪量均低于油酸对照组。相对于油酸组,12周后癸酸组空腹血明显降低(P<0.05);在葡萄糖耐量试验中,与油酸组比较,癸酸组在给予葡萄糖后0.5h血糖明显降低(P<0.05);给予葡萄糖1h和2h后癸酸组血糖仍然较低,与油酸组比较差异明显(P<0.05),癸酸组血糖曲线下面积明显低于油酸对照组。结论在能量摄入相同前提下,中链脂肪酸辛酸和癸酸都能降低高脂肪饲料导致的体重增加和体脂肪量,降低糖尿病kkay小鼠空腹血糖,此外癸酸还能改善葡萄糖耐量。

Objective To observe the effect of medium-chain caprylic and capric acids on glucose metabolism in Kkay mice with diabetes mellitus（DM）. Methods Thirty-six male Kkay mice with DM were randomly divided into high fat diet ＋ caprylic acid（C8） group, high fat ＋ capric acid（C10） group, and high fat ＋ oleic acid（OA） group（12 in each group）, and fed with the diets for 12 weeks. Then, their body weight, diet consumption and blood glucose were observed. Results Twelve weeks after fed with high fat diet, the body weight, body gain and body fat were significantly lower in C8 and C10 groups than in OA group（P〈0.05）, and the fasting blood glucose level was significantly lower in C10 group than in OA group（P〈0.05）. Blood glucose tolerance test showed that the blood glucose level was significantly lower in C8 group than in OA group 30rain, 1 and 2h after oral administration of glucose（P〈0.05）. The AUC was significantly lower in C10 group than in OA group（P〈0.05）. Conclusion Caprylic and capric acids can reduce body weight gain, body fat, and fasting blood glucose level in DM Kkay mice fed with high fat diet, and capric acid can improve their blood glucose tolerance.