芭尔糖及其组分对小鼠餐后血糖和胰岛素的影响

Effect of parsweet sugar and its key component on postprandial glucose and insulin levels in mice

目的观察芭尔糖和其主要成分赤藓糖醇对昆明小鼠和KKay糖尿病小鼠糖代谢的影响。方法将昆明小鼠和KKay糖尿病小鼠各按空腹血糖随机分为2组,每组15只,分别进行以下实验:1)葡萄糖耐量实验:赤藓糖醇组给予赤藓糖醇2mg/g+葡萄糖2mg/g,对照组仅给予葡萄糖2mg/g;2)淀粉耐量实验:赤藓糖醇组给予赤藓糖醇2mg/g+淀粉2mg/g,对照组仅给予淀粉2mg/g。比较各时间点血糖和血糖曲线下面积;3)餐后胰岛素水平测定实验:芭尔糖组给予芭尔糖2mg/g+淀粉2mg/g,赤藓糖醇组给予赤藓糖醇2mg/g+淀粉2mg/g,对照组给予淀粉2mg/g,测定灌胃后1h血胰岛素水平。结果赤藓糖醇组与葡萄糖组比较,无论正常小鼠还是糖尿病小鼠,餐后0.5h的血糖较对照组都有明显下降。赤藓糖醇组与淀粉组比较,正常小鼠餐后0.5h血糖和糖尿病小鼠餐后1h血糖有明显降低作用,无论正常小鼠还是糖尿病小鼠,赤藓糖醇餐后血糖曲线下面积较淀粉组明显下降。与淀粉组比较,芭尔糖组和赤藓糖醇组对昆明小鼠和KKay小鼠餐后1h血胰岛素的分泌作用均无统计学差异。结论芭尔糖及其主要组分不升高餐后血糖,甚至降低餐后血糖和血糖曲线下面积,不增加胰岛素分泌。

Objective To observe the effect of parsweet sugar and its key component, erythritol, on glucose metabolism in Kunming and Kkay mice with diabetes mellitus（DM）. Methods Kunming and Kkay mice with DM were randomly divided into experiment group and control group according to their fasting blood glucose （15 in each group）. Mice in experiment group were given 2mg/g erythritol and 2mg/g glucose, or 2mg/g erythritol and 2mg/g starch, while those in control group were given only 2mg/g glucose or only 2mg/g starch. Blood glucose level and area under blood glucose curve at different time points were compared. Postprandial insulin levels were measured in mice after 2mg/g parsweet sugar and 2mg/g starch or 2mg/g erythritol and 2mg/g starch were given, while those in control group were given only 2mg/g starch. Blood insulin level lh after gavage was detected. Results The blood glucose levels were significantly lower in normal and DM mice than in controls 30 minutes after erythritol and glucose were given, or 30 minutes after erythritol was given and 1 hour after starch was given. The area under blood glucose curve was significantly lower in normal and DM mice after erythritol was given than after starch was given. No significant difference in blood insulin level was found in Kunming and Kkay mice 1 hour after parsweet sugar, erythritol and starch were given. Conclusion Parsweet sugar and its key component, erythritol, can decrease the postprandial blood glucose and insulin levels and even the area under blood glucose curve after meal.