去细胞尿道修复大鼠尿道缺损

Repair of urethral defect in rats with acellular urethra

目的探讨化学去细胞尿道修复尿道缺损的效果。方法取SD大鼠尿道20根,每段长1cm,采用化学去细胞法处理大鼠尿道的细胞成分,保留细胞外基质,制备去细胞尿道。将10只Wistar大鼠尿道制成1cm整段缺损模型,用SD大鼠去细胞同种异体尿道移植修复大鼠尿道的整段缺损。术后3周进行大鼠尿动力学检测及VG染色。另取10只Wistar大鼠异体移植及10只Wistar大鼠自体移植作对照。结果与对照组相比,处理组术后尿道压力、膀胱最大容积和残余尿量差异无统计学意义(P>0.05)。VG染色结果显示,去细胞处理组吻合口断端被染成红色,肌纤维排列整齐分布均一,再生肌纤维长入远端尿道。异体移植对照组肌纤维形态均一,肌纤维排列整齐分布均一未见肌纤维长入远端尿道。结论化学去细胞同种异体尿道,可修复大鼠尿道损伤。

Objective To study the repair of urethral defect in rats with acellular urethra in order to find its ideal methods and materials. Methods Twenty urethras were taken from SD rats and cut into lcm-long segments. Their cell components were isolated with chemical acellular methods with their extracellular matrix preserved for the preparation of acellular urethra. A lcm-long segment defect model of urethra from 10 Wistar rats was established. Urethral defect in SD rats was repaired with acellular allo-urethra transplantation. Uriodynamics was detected 3 weeks after operation with VG staining. Ten Wistar rats underwent allo-transplantation and another 10 Wistar rats that underwent auto-transplantation served as controls. Results No difference was observed in urethral pressure, maximal bladder volume and residual urine volume between experimental and control groups（P〉0.05）. VG staining showed that the broken end of anastomotic stoma was stained red in experimental group. The muscle fibers were orderly arranged and well distributed. The regenerated muscle fibers grew into the distal urethra. The muscle fibers were also orderly arranged and well distributed in control group with no regenerated muscle fibers grown into the distal urethra. Conclusion Acellular allo-urethra can repair the urethral defect in higher mammals.