海藻溴酚化合物对糖尿病大鼠肾超微结构及结缔组织生长因子蛋白表达的影响

Effects of seaweed bromophenol compounds on ultrastructure and protein CTGF expression of diabetic rats renal tissues

目的研究海藻溴酚化合物对糖尿病肾损伤大鼠结缔组织生长因子(CTGF)蛋白表达的影响。方法采用STZ注射制作糖尿病大鼠模型,随机分为对照组、模型组、化合物A和化合物B剂量组(0.5,10mg.kg-1),灌胃给药12周。处死大鼠,测血浆及肾组织中谷胱甘肽过氧物酶(GSH-Px)的活力及丙二醛(MDA)的含量;透射电镜法观察大鼠肾组织的病理改变;免疫组化法检测CTGF蛋白的表达。结果与对照组相比,模型组血浆及肾组织匀浆中GSH-Px活力下降,MDA含量升高。各干预组中GSH-Px的活力较模型组有升高的趋势,MDA含量有下降趋势。各干预组的CTGF平均吸光度值低于模型组。电镜下各干预组肾小球及肾小管病变较模型组减轻。结论海藻溴酚化合物A、B能提高糖尿病大鼠机体抗氧化水平,并能一定程度改善肾脏的病理变化,但其具体机制有待进一步探讨。

Objective To study the effect of seaweed bromophenol compounds on the expression of CTGF in kidney damage of diabetic rats. Methods Diabetic model was induced by a single intraperitoneal injec- tion of streptozoticin（STZ） to male Wistar rats and randomly divided into control group, diabetic group, compound A and compound B （low-dose groups and hlgh-dose groups. Rats in test groups were given compound A and B at doses of 5,10 mg·kg^-1 dlig for 12 weeks. Alter 12 weeks, the activities of glutathione peroxidase （GSH-Px） and the malonic dialdehyde （MDA） contents in plasma and kidneys were determined. The expression o[ CTGF in rats＇ renal tissues was determined by immuohistochemis-try method and the histopathology rat changes were observedunder the electron microscopy. Results Compared with control group, the GSH-Px activities in the plasma and kidney tissue decreased, while MDA contents increased significantly in diabetic group. GSH-Px activities tend to increase and MDA contents were downward trend in intervention groups than those in diabetic group. The intervention groups of CTGF mean optical density value lower than the diabetic group, electron microscopy of glomerular and tubular lesions reduce in the intervention groups compared with diabetic group also the high-dose group compared with the low dose group. Conclusion Seaweed bromophenol compounds A and B could improve the body＇s antioxidant levels in diabetic rats, and may improve the renal pathologi- cal changes. But the specific mechanism remains to be further explored.