大麦黄矮病毒运动蛋白核定位信号对PVX病毒运动的影响

Effect of BYDV-MP Nuclear Localization Signal on the Movement of PVX

应用马铃薯X病毒(PVX)载体研究大麦黄矮病毒运动蛋白(BYDV-MP)核定位信号对PVX病毒运动的影响。我们将BYDV-MP克隆到PVX改造载体pGR107中,同时用GFP作为指示蛋白,研究BYDV-MP对异源病毒PVX系统运动的影响。侵染烟草发现BYDV-MP能够在PVX载体中表达并能加强病毒的系统侵染;将PVX编码系统运动蛋白25kD基因进行缺失突变,重复上述试验发现BYDV-MP能够补偿PVX系统运动;将BYDV-MP的N端的第五、六位氨基酸和第七位氨基酸进行替换突变,侵染烟草发现BYDV-MP的N端的第五、六位氨基酸突变不能完全抑制PVX系统运动,但是可以延迟并减弱PVX系统运动;BYDV-MP的N端的第七位氨基酸突变能够完全抑制PVX系统运动。

By using PVX derived vector pGR107,the effect of BYDV-MP nuclear localization signal on the movement of PVX was studied.BYDV-MP was cloned into pGR107 using GFP as an indicator.BYDV-MP was then shown to induce the systemic infection and exacerbate the symptom of PVX through infecting Nicotiana benthamiana.When the PVX gene encoding 25kD protein,which functioned as a systematic movemnet protein,was deleted and the above experiment was repeated,the result showed that BYDV-MP could compensate the systemic movement of PVX.A serial mutants with substitutions on the fifth,sixth and seventh amino acids of BYDV-MP nuclear localization signal was further constructed.It was found that the mutants at the fifth,sixth amino acids in BYDV-MP nuclear localization signal could only delay or weaken systemic movement of PVX whereas the mutant at seventh amino acid could entirely inhibit systemic movement of PVX.