卵巢上皮癌中细胞外信号调节激酶的表达及其与预后的关系

Clinical significance and prognostic value of extracellular signal-regulated kinases in epithelial ovarian cancer

目的:探讨磷酸化细胞外调节激酶(p-ERK)在卵巢上皮癌组织中的表达及其与肿瘤患者预后之间的关系。方法:应用Western印迹方法分别测定正常卵巢组织、良性卵巢组织和卵巢上皮癌组织中p-ERK的含量;收集卵巢上皮癌患者的临床资料;应用Kaplan-Meier法和Cox回归对卵巢上皮癌患者进行生存分析。结果:(1)卵巢上皮癌组织中的p-ERK含量明显高于良性卵巢组织和正常卵巢组织(P<0.01),Ⅲ～Ⅳ期卵巢上皮癌组织中p-ERK含量明显高于I～II期(P<0.01),良性卵巢组织中p-ERK含量与正常卵巢组织相比无显著差异(P>0.05);(2)低分化卵巢上皮癌组织中的p-ERK含量高于中、高分化(P<0.01);(3)在不同病理类型卵巢癌中,与浆液性卵巢上皮癌组织相比,未分化腺癌组织中p-ERK含量与其相仿(P>0.05),而黏液性卵巢上皮癌和卵巢子宫内膜样癌组织中p-ERK含量较低(P<0.05);(4)p-ERK含量与生存时间呈负相关,p-ERK含量高的卵巢上皮癌患者生存时间短;(5)卵巢上皮癌患者的生存时间与p-ERK含量、残余灶、肿瘤分期、肿瘤分级以及化疗的疗程有着密切关系(P<0.05),而与肿瘤的病理类型没有相关性(P>0.05)。结论:p-ERK可能是卵巢上皮癌发病机制一个重要的调节激酶;测定p-ERK水平可能对卵巢上皮癌患者预后有预测价值。

AIM： To investigate the expression of phosphorylated extracellular signal-regulated kinase（p-ERK） in epithelial ovarian cancer and to analyze the correlation between the expression of p-ERK and prognosis of epithelial ovarian cancer patients.METHODS： The samples of normal ovarian tissues,benign ovarian tissues and ovarian carcinoma were collected.The expression of p-ERK in each sample was detected by Western blotting.Assemble clinical data and survival were analyzed with Kaplan-Meier method and Cox regression.RESULTS： The expression level of p-ERK in ovarian carcinoma was significantly higher than that in benign ovarian tissues and in normal ovarian tissues.The overexpression of p-ERK protein was significantly higher in stage Ⅲ/Ⅳ disease than that in stage I/II disease.No difference of p-ERK expression level between the benign ovarian tissues and the normal ovarian tissues was observed.The overexpression of p-ERK was found more often in poorly-differentiated ovarian cancer.Compared with serous carcinoma of ovary,the expression level of p-ERK was similar in undifferentiated adenocarcinoma.The expression level of p-ERK was low in endometrioid carcinoma of ovary and mucinous carcinoma of ovary.The expression level of p-ERK was negatively correlated with the survival time.Increased p-ERK expression was strongly correlated to the poor survival.Shorter survival time was significantly associated with p-ERK expression,residual tumor,stage,grade and chemotherapy course.Shorter survival time was not significantly correlated with tumorous pathology.CONCLUSION： p-ERK is a key regulation kinase involved in epithelial ovarian cancer.The expression level of p-ERK seems to have a prognosis value in epithelial ovarian cancer.