转基因在苹果蠹蛾不育昆虫释放技术中的应用

Transgenic approaches applied to the sterile insect technique of codling moth control

使用不育昆虫释放技术是一项新兴的苹果蠹蛾Cydia pomonella(L.)防治方法,使用转基因得到不育雄蛾具有比传统辐射方法更多的优点。转基因技术通过使用基于piggyBac等转座子的质粒载体,并插入显性条件致死基因以培育遗传性别品系,同时插入荧光蛋白等标记基因来显示转基因的效果;在人工控制的发育条件,建立成熟的稳定苹果蠹蛾品系,用以最终的田间释放以达到防治的目的。

The sterile insect technique （SIT） is a relatively new approach to controlling the codling moth. Compared with the traditional irradiation method, the application of transgenic techniques is much better at producing sterile males. In addition to manual control of developmental conditions this involves using plasmid vectors based on the piggyBac transposon, inserting a dominant conditional lethal gene to culture genetic sexing strains and inserting marker genes such as fluorescent proteins to elucidate the efficacy of gene transfer.