棉铃虫活化的蛋白激酶C受体1(RACK1)基因的分子鉴定

Molecular identification of Helicoverpa armigera receptor for activated C kinase 1 gene

活化的蛋白激酶C受体l(receptor for activated C kinase1,RACKl)广泛分布于真核生物和原核生物中,在生物体内具有极其重要的调节功能。本实验利用RT-PCR和RACE的方法扩增获得了棉铃虫Helicoverpa armigera (Hübner)RACK1基因全序列,序列分析结果表明,该基因开放阅读框为957bp,编码319个氨基酸残基。5'端非编码区长为36bp,3'端非编码区长为112bp。发育时相表达发现RACK1基因在棉铃虫的蜕皮时期大量表达,进一步的激素处理实验发现,蜕皮激素诱导RACK1基因表达,保幼激素和饥饿抑制RACK1基因表达。这些研究结果为进一步研究RACK1基因的功能奠定基础。

The receptor for activated C kinase 1 （RACK1） has an important regulatory function and is ubiquitous in plants and animals. The receptor for activated C kinase 1 cDNA of Helicoverpa armigera （Hübner） was isolated using the reverse transcription polymerase chain reaction （RT-PCR） and rapid amplification of cDNA ends （RACE）. Nucleotide sequence analysis revealed that the open reading frame （ORF） of RACK1 was 957 bp in size and encoded 319 amino acid residues. A 3′untranslated region （UTR） of RACK1 contained 112 nucleic acids and a 5′ UTR contained 36 nucleic acids. Temporal expression analysis found that RACK1 was up-regulated during molting. Hormone-challenge experiments revealed that RACK1 was up-regulated by molting hormones and inhibited by a juvenile hormone analogue and starvation. These results provide a fountain for further study of the function of RACK1.