mtCOI PCR-RFLP技术鉴别中国入侵型和土著型烟粉虱种群的有效性分析

Utility of mtCOI PCR-RFLP in differentiating invasive and local whitefly biotypes of China

烟粉虱Bemisia tabaci (Gennadius)是世界范围内最重要的入侵生物之一,准确地鉴别烟粉虱入侵生物型和土著生物型具有十分重要的现实意义。mtCOI PCR-RFLP技术具有快速、高效的特点,是当前应用最广泛的烟粉虱生物型鉴定技术,但是,不同限制性内切酶为基础的mtCOI PCR-RFLP技术在鉴定我国烟粉虱种群中的有效性仍不明了。本文比较了已报导的5种mtCOI PCR-RFLP技术在鉴别中国烟粉虱种群入侵生物型和土著生物型(B型、Q型,ZHJ1型、ZHJ2型、ZHJ3型)的有效性。结果表明,内切酶AluI不能区分B型和ZHJ2型烟粉虱;内切酶TaqI不能准确区分ZHJ3型和Q型烟粉虱个体;而内切酶VspI不仅不能准确区分ZHJ1型和Q型烟粉虱个体,也不能准确区分B型和ZHJ2型烟粉虱;内切酶MseI和Tru9I则不能有效鉴别上述5种烟粉虱生物型,因此不适宜推广使用。

Bemisia tabaci （Gennadius） is one of the most important invasive pests in China. The mtCOI PCR-RFLP technique is currently widely used to identify different whitefly biotypes worldwide. In this paper, we compared the utility and efficiency of five commonly reported mtCOI based PCR-RFLP techniques in differentiating invasive and local B. tabaci biotypes （B, Q, ZHJ1, ZHJ2 and ZHJ3） in China. Our results show that the AluI enzyme fails to differentiate between the B and ZHJ2 biotypes, the TaqI enzyme cannot effectively differentiate between the ZHJ3 and Q biotypes, the VspI enzyme is not effective in differentiating between either the B and ZHJ2 biotypes or the Q and ZHJ1 biotypes, and the MseI and Tru9I enzymes are completely unable to identify any of the five biotypes found in China.