三种抗生素对B型和Q型烟粉虱内共生菌的去除效果比较研究

A comparative study of the removal of endosymbionts in Bemisia tabaci biotypes B and Q using three antibiotics

利用烟粉虱Bemisia tabaci(Gennadius)内共生菌特异性引物,研究了内共生菌在B、Q型烟粉虱种群中的分布和感染率,同时评价了3种不同的抗生素利福平、氨苄青霉素和硫酸卡那霉素分别在3种不同的浓度下(100.0、50.0及25.0μg/mL)对烟粉虱内共生菌的去除效果。结果表明:B、Q型烟粉虱原生内共生细菌Portiera的带菌率均为100.0%;B、Q型烟粉虱次生内共生菌Hamiltonella的带菌率分别为91.7%和100.0%;B型烟粉虱次生内共生菌Rickettsia的带菌率为87.5%,Q型为0;其它次生内共生菌在B、Q型烟粉虱中均未检测到。利福平、氨苄青霉素和硫酸卡那霉素在3种不同的浓度下均不能去除B、Q型烟粉虱Portiera;利福平、氨苄青霉素在3种不同的浓度下均能完全去除B型烟粉虱Rickettsia,硫酸卡那霉素在不同浓度下去除Rickettsia的效果不同;3种抗生素去除Hamiltonella的能力受抗生素种类以及浓度的影响。同一抗生素在不同浓度下去除Hamiltonella的效果均是100.0μg/mL>50.0μg/mL>25.0μg/mL;不同浓度的抗生素去除Hamiltonella的效果均是利福平>氨苄青霉素>硫酸卡那霉素,各浓度与各抗生素之间的去除Hamiltonella的效果均具有显著性差异。

A PCR survey of endosymbionts in one B and one Q Bemisia tabaci biotype was conducted. Kanamycin sulfate, ampicillin trihydrate, and rifampicin were used to investigate the sensitivity of endosymbionts in the two biotypes to antibiotics. The results show that 100% of all individuals of the both biotypes had Portiera. Hamiltonella was also found in both biotypes, with an infection frequency of 91.7% and 100.0%, respectively. Rickettsia was only detected in 87.5% of the B biotype. Other endosymbionts including Wolbachia, Fritschea, Arsenophonus, and Cardinium were not detected in either biotype. The three antibiotics failed to eliminated Portiera from any individual of the B and Q biotypes. Rickettsia of the B biotype was selectively eliminated by kanamycin sulfate in a dose-dependent manner. Hamiltonella was also eliminated by all three antibiotics from both biotypes. The ease of elimination depended on the endosymbiont species, dosage and the interaction between these two factors. Rifampicin was superior to ampicillin and kanamycin sulfate in eliminating Hamiltonella and high dosages were more effective than low dosages of the same antibiotic.