黄金锦络石组织培养快繁技术体系的建立

Establishment of Tissue Culture and Rapid Propagation Technique System for Trachelospermum asiaticum

以黄金锦络石带顶芽或腋芽的半木质化茎段为外植体,系统研究了外植体灭菌方法,生长素和分裂素的不同种类和浓度对无菌芽苗增殖和生根的影响,建立了组培快繁技术体系。结果表明:外植体灭菌乙醇45 s,HgCl2处理10 min较为适宜;增殖培养基为MS+BA 1.5～3.0 mg/L和NAA 0.2 mg/L,增殖系数最高,达到7.9;生根培养基1/2MS+IBA 0.05 mg/L+NAA 0.15 mg/L,生根率可达90%多,根数为每株6～7条,平均根长在3 cm多,而且还有侧根生出。

Taking the semi-lignified stem section with top buds or axillary buds of Trachelospermum asiaticum ＇Ougonnishiki＇ as explant,the effects of explant sterilization method and different kinds and concentrations of plant growth regulators on the multiplication and rooting of no-virus bud-plantlets were systematically studied,and the tissue culture and rapid propagation technique system was established.The results showed that it was appropriate for the explant to disinfect in ethanol for 45 seconds and in HgCl2 for 10 minutes.The optimum medium for multiplication was MS＋ BA 1.5~3.0 mg/L＋ NAA 0.2 mg/L,and it obtained the highest multiplication coefficient（7.9）.The optimum medium for rooting was 1/2MS＋ IBA 0.05 mg/L＋ NAA 0.15 mg/L,the rooting rate was more than 90%,the root number was 6~7 per plant,the average root length was above 3 cm,and some lateral roots grew out.