软骨素酶ABC去除化学去细胞神经中硫酸软骨素蛋白多糖

Degradation of Chondroitin Sulfate Proteoglycan of Chemically Extracted Acellular Nerve by Chondroitinase ABC

目的:观察软骨素酶ABC(ChABC)是否能降解化学去细胞神经(CEAN)中的硫酸软骨素蛋白多糖(CSPG)。方法:取SD大鼠的坐骨神经,按化学法制备去细胞神经,ChABC浸泡16h,行HE染色、CSPG及基底膜素层粘连蛋白(LN)免疫组化染色,观察去细胞神经的结构及成分变化。Wistar大鼠15只,切除左坐骨神经5mm,随机分为A、B、C 3组各5只,分别采用自体神经、CEAN、经ChABC处理的CEAN修复缺损,术后15d测量各组神经纤维生长情况。结果:ChABC处理去细胞神经后,CSPG免疫组化染色示CSPG表达显著降低;HE染色显示结构无明显变化;LN免疫组化染色无明显变化。术后2周A组神经再生距离长于B、C组(P<0.05),C组优于B组(P<0.05)。结论:ChABC可降低化学去细胞神经中的CSPG,有利于促进神经再生。

Objective： To observe the degradation of Chondroitin sulfate proteoglycan（CSPG） of chemically extracted acellular nerve（CEAN） by Chondroitinase ABC.Methods： The CEAN was prepared from the sciatic nerve of rats which were then degraded by Chondroitinase ABC and dyed by HE staining.CSPG and Laminin immunohistochemical staining were performed to evaluate the structure and ingredient of CEAN after degrading CSPG from CEAN.Fifteen Wister rats were randomly divided into three groups（n=5 in each group）,the autograft group（A group）,acellular nerve allograft group（B group） and acellular nerve treated with Chondroitinase ABC allograft group（C group）.A 10-mm gap was bridged with autografing,chemically extracted acellular nerve allograft and chemically extracted acellular nerve treated with Chondroitinase ABC respectively.The nerve regeneration length of 3 groups was measured on day 15 after operation.Results： The expression of CSPG in the CEAN was decreased significantly after being treated with Chondroitinase ABC whereas no significant changes of the structure of the CEAN was detected by HE or Laminin immunohistochemical staining.The length of nerve regeneration in A group was longer than that in other groups（P0.05） while the nerve regeneration in C group was significantly improved relative to that in B group（P0.05）.Conclusion： Chondroitinase ABC treatment can decrease CSPG and improve the speed of peripheral nerve regeneration repaired with CEAN.