苦瓜种子蛋白的双水相提取及抑菌性研究

Extraction of Seed Protein from Momordica charantia by Aqueous Two-phase System and Its Antibacterial Effect

筛选优化了聚乙二醇(PEG)/硫酸铵双水相的质量分数、分配系数、回收率等影响因素,得到了苦瓜种子蛋白的最佳萃取条件:在26%硫酸铵、22%聚乙二醇条件下,分配系数最小,达0.089,回收率为96%,得到分子量为35.6 kD和12.3kD的苦瓜纯化蛋白。抑菌试验表明,苦瓜种子蛋白对细菌、真菌均有不同程度的抑制作用,且纯化蛋白的抑菌性大于粗提蛋白。

Through screening and optimizing some influential factors,such as the mass fraction of polyethylene glycol（PEG） / ammonium sulfate aqueous two-phase,partition coefficient and recovery rate,the best conditions for the extraction of seed protein from balsam pear（Momordica charantia） were obtained.The result revealed that： when the mass fraction of ammonium sulfate and PEG was 26% and 22% respectively,the partition coefficient was the lowest（0.089）,the recovery rate was the highest（96%）,and the purified seed protein with molecular weight 35.6 kD and 12.3 kD was acquired from Momordica charantia by aqueous two-phase system.The antibacterial tests indicated that Momordica charantia seed protein had various antibacterial effects to bacterium and fungus,and the antibacterial effect of the purified protein was better than that of the crude protein.