携带四环素耐药基因的鸭源大肠杆菌质粒不相容群分析

Analysis of Incompatible Groups of Plasmids in Duck-derived Escherichia coli Carrying Tetracycline Resistant Genes

目的:用PCR方法测定22株携带四环素耐药基因鸭大肠杆菌所含质粒的不相容群。方法:设计18对引物,分为5个多重PCR体系和3个单一PCR体系,用来检测识别FIA、FIB、FIC、HI1、HI2、I1、L/M、N、P、W、T、A/C、K、B/O、X、Y、F和FⅡ复制子。结果:22株鸭大肠杆菌中,分别有7、4、9、2、6、2、3、7株和19株属于IncI1、IncN、IncFIB、IncY、IncP、IncFIC、IncA/C、In-cK和IncFⅡ。试验菌株所含质粒大部分属于IncFⅡ,占86.4%,属于1、2、3、4、5个不相容群组合的分别为13.6%、31.8%、31.8%、18.2%和4.5%。

Objective： The incompatible groups of the plasmids carrying tetracycline resistant genes in 22 duck Escherichia coli isolates were determined by using PCR method.Methods： 18 pairs of designed primers were divided into five multiple PCR systems and three single PCR systems,and they were used to detect FIA,FIB,FIC,HI1,HI2,I1,L/M,N,P,W,T,A/C,K,B/O,X,Y,F and FⅡ replicons.Results： seven,four,nine,two,six,two,three,seven and nineteen of 22 E.coli isolates from duck belonged to IncI1,IncN,IncFIB,IncY,IncP,IncFIC,IncA/C,IncK and IncFⅡ,respectively.Most（86.4%） of plasmids in the tested strains belonged to IncFII.One incompatible group and 2,3,4 and 5 incompatible group combinations accounted for 13.6%,31.8%,31.8%,18.2% and 4.5%,respectively.