Hp感染与胃癌组织中Bcl-2、Bax表达的相关性研究

Association of Hp infection and the expression of Bcl-2,Bax in gastric carcinoma

目的:揭示Bcl-2,Bax在胃癌组织的表达与Hp感染的相关性。方法:采用快速尿素酶试验及组织学改良Giemsa染色法联合检测130例胃癌组织与70例慢性胃炎组织中Hp感染情况,免疫组织化学PV9000法,检测130例胃癌组织与70例慢性胃炎组织中Bcl-2,Bax蛋白的阳性表达情况。结果:Hp(+)组Bcl-2蛋白的阳性表达率明显高于Hp(-)组(P<0.01);胃癌组织中Hp感染与Bcl-2蛋白表达之间存在正相关关系(P<0.01,r=0.288);Hp(+)组Bax蛋白的阳性表达率明显低于Hp(-)组(P<0.01);胃癌组织中Hp感染与Bax蛋白表达之间存在负相关关系(P<0.01,r=-0.536)。结论:Hp感染与Bcl-2蛋白表达存在正相关性,与Bax蛋白表达存在负相关性,提示Hp感染与细胞凋亡,二者可能共同参与胃癌的发生发展过程。

Objective：To reveal the correlation between the expression of Bcl-2,Bax in gastric carcinoma tissues and Hp infection.Methods： Tissue samples were collected from 130 patients with gastric carcinoma and 70 patients with chronic gastritis,Hp was identified by rapid urease test and modified Giemsa-staining.Immunohistochemical method（PV9000）was used to detect the expression of Bcl-2,Bax.Results： Bcl-2 protein expression of Hp（＋）group was significantly higher than Hp（-）group（P0.01）;Hp infection and the expression of Bcl-2 protein in gastric carcinoma tissue was positively correlated（P0.01,r=0.288）;Bax protein expression of Hp（＋）group was significantly lower than Hp（-）group（P0.01）;Hp infection and the expression of Bax protein in gastric carcinoma tissue was negatively correlated（P0.01,r=-0.536）.Conclusion： Hp infection and the expression of Bcl-2 protein was positively correlated,bat negatively correlated with the expression of Bax protein negative correlation,Hp infection and cell apoptosis were involved in the development and progression of gastric carcinoma.