白藜芦醇对乳腺癌MDA-MB-231细胞凋亡及Bcl-2蛋白表达的影响

Resveratrol induces the apoptosis of breast cancer MDA-MB-231 cells and inhibits the expression of Bcl-2

目的探讨白藜芦醇(resveratrol,Res)对人乳腺癌MDA-MB-231细胞凋亡及相关蛋白Bcl-2表达的影响。方法不同浓度白藜芦醇处理人乳腺癌MDA-MB-231细胞,MTT法检测白藜芦醇对细胞增殖抑制率,流失细胞技术检测细胞凋亡,Western blot法检测细胞凋亡相关蛋白Bcl-2、Bcl-xl、Mcl-1和Caspase-3的表达。结果白藜芦醇25、50、100、200μmol/L处理细胞,增殖抑制率分别为(24.22±1.66)%、(30.79±1.60)%、(45.72±1.25)%和(53.31±1.94)%。白藜芦醇0、50、100、200μmol/L处理细胞,凋亡率分别为(3.53±0.25)%、(28.80±1.34)%、(38.78±1.19)%和(58.96±1.26)%。Western blot结果显示,白藜芦醇处理细胞后,Caspase-3蛋白裂解片段表达增加,凋亡相关蛋白Bcl-2和Bcl-xl的表达减低。结论白藜芦醇能诱导MDA-MB-231细胞凋亡增加,这可能与白藜芦醇激活Caspase-3蛋白同时抑制凋亡相关蛋白Bcl-2和Bcl-xl表达有关。

Objective To investigate the effect of resveratrol on the proliferation and apoptosis of the breast cancer cell line MDA-MB-231 and the expression of Bcl-2.Methods Human breast cancer cell line MDA-MB-231 cells was treated with resveratrol at different concentrations,MTT assay was used to detect the cell proliferation and flow cytometry was applied to analyze cell apoposis.The expressions of Caspase-3,Bcl-2,Bcl-xl,Mcl-1 were analysed by Western blot.Results Resveratrol inhibited the growth of MDA-MB-231 cells in a cocentration dependent manner.The inhibiting rates of the cells were（24.22±1.66）%,（30.79±1.60）%,（45.72±1.25）% and（53.31±1.94）% respectively,by different concentrations of resveratrol（25,50,100 and 200μmol/L）.Annexin V-FITC/PI binding assay showed that resveratrol induced apoptosis in MDA-MB-231 cells,the apoptosis rates of the four groups（0,50,100,200μmol/L） were（3.53±0.25）%,（28.80±1.34）%,（38.78±1.19）% and（58.96±1.26）%,respectively.Western blot showed that resveratrol inhanced the activation of Caspase-3 and inhibited the expression of Bcl-2 and Bcl-xl.Conclusion Resveratrol can inhance the apoptosis of MDA-MB-231,which might be related to inhibition of the expression of Bcl-2 and Bcl-xl and to inhancing the activation of Caspase-3.