β-蜕皮甾酮对匹罗卡品致痫小鼠海马神经元凋亡相关基因表达的影响

Effect of 20-hydroxyecdysone on hippocampal neurons apoptosis-associated genes expression in mice with pilocarpin induced epilepticus

目的观察β-蜕皮甾酮(20-hydroxyecdysone)对小鼠癫痫持续状态后海马神经元凋亡及凋亡相关基因的影响。方法将30只CD-1小鼠随机分成正常组、造模组、β-蜕皮甾酮治疗组。用锂-匹罗卡品腹腔注射法诱导小鼠癫痫持续状态,造模成功后72h,采用尼氏染色观察各组小鼠海马区神经元数目和形态的变化,免疫组化法和Westernblot法检测各组神经元凋亡及凋亡相关基因Bcl-2,Bax的表达。结果模型组小鼠CA1区部分细胞肿胀,锥体细胞排列紊乱,细胞形态不规则,药物治疗组较模型组细胞数目增多,细胞排列有所改善。免疫组化和Westernblot结果可见细胞凋亡因子Bax表达模型组高于正常对照组,药物治疗组低于模型组;抑制细胞凋亡因子Bcl-2表达模型组高于正常对照组,药物治疗组高于模型组。结论β-蜕皮甾酮(20-HE)可能通过降低Bax,提高Bcl-2蛋白的表达,减轻癫痫发生后对大脑的损害。

Objective To observe the effect of 20-hydroxyecdysone on hippocampal neurons apoptosis and the expression of associated genes Bcl-2,Bax in mice with status epilepticus（SE）.Methods Thirty CD-1 mice were randomized into normal group,model group,and 20-hydroxyecdysone therapy group.Mouse models with SE were established by pilocarpine injection.Seventy-two hours after the establishment of the models,hippocampal neurons apoptosis and the expression of associated genes Bcl-2,Bax in three groups were observed by immunohischemical method and Western blot.Results Bax protein expression levels were increased in model group than in normal group,but decreased in 20-hydroxyecdysone group than in model group.Bcl-2 protein expression levels were increased in model group than in normal group and decreased in 20-hydroxyecdysone group than in model group.Conclusion Downregulation of Bax and upregulaiton of Bcl-2 protein expression might be related to recovery of cerebral damaged neurons by 20-hydroxyecdysone.