摘要
目的研究重组人促红细胞生成素(recombinant human erythropoietin,rHuEPO)对急性脊髓损伤大鼠血管内皮生长因子(vascular endothelial growth factor,VEGF)表达的影响。方法参照Nystrom's压迫方法制作大鼠脊髓压迫损伤模型,成年健康Wistar大鼠72只,雌雄不限,按随机数字表法分为正常对照组8只、损伤组32只、重组人促红细胞生成素治疗组32只。免疫组化和Western blot检测各组大鼠VEGF的表达。结果免疫组化结果显示:损伤组VEGF阳性产物的平均光密度值(mean optic density,MOD)显著高于正常对照组(<0.01),rHuEPO治疗组与损伤组相比MOD值明显升高(<0.01)。Western blot结果显示:与正常对照组比较,损伤组VEGF积分光密度值(integrated density value,IDV)与内参照IDV的比值明显升高(<0.01),而rHuEPO治疗组则明显高于损伤组(<0.01)。结论 rHuEPO参与脊髓继发性损伤修复,可能与上调VEGF的表达相关。
Objective To study the expression of vascular endothelial growth factor（VEGF） in acute spinal cord injury rat after recombinant human erythropoietin（rHuEPO）was injected.Methods The spinal cord injury was induced with Nystrom＇s way.The healthy adult Wistar rats（72） were randomly divided into normal control group,spinal cord injury group,recombinant human erythropoietin treated group on average.The expression of VEGF was observed by immunochemistry and Western blot methods in all groups.Results The mean optic density（MOD） of VEGF positive product increased significantly in acute spinal cord injury group than in normal control（0.01）,and increased in the recombinant human erythropoietin treated group than in acute spinal cord injury group（0.01） by immunochemistry.The integrated density value（IDV） for VEGF protein band increased significantly in the spinal cord injury group than in normal control（ 0.01）,and increased in recombinant human erythropoietin group than in spinal cord injury group（0.01） by Western blot.Conclusion The involvement of recombinant human erythropoietin in the recovery of acute spinal cord injury might be related to the upregulation of the expression of VEGF.
出处
《解剖科学进展》
CAS
2012年第1期70-73,共4页
Progress of Anatomical Sciences
基金
国家自然科学基金资助项目(No.81100924)
