超声处理对人参种子萌发以及SOD、POD的影响

Influence of the Ultrasonic Treatment on the Preliminary Study of Ginseng Seeds and the Influence of SOD,POD

目的:人参种子具有双重休眠的特点,要经过一定时间的形态后熟与生理后熟才能萌发。影响种子萌发内在条件是胚的完整性,其内在的酶系统及生化反应也是影响种子发芽的重要生理指标。其中SOD(超氧化物歧化酶)、POD(过氧化物酶)直接影响种子活力。方法:通过超声波处理来探索对人参种子萌发的影响。结果:超声温度50℃时,裂口率为90%,高于CK13百分点;时间30min时,裂口率为90%,高于CK13百分点;功率550W时,裂口率为95%,高于CK18百分点。而超声温度为30℃时,胚率是66.6%,高于CK6.0百分点;时间为20min时,胚率是65.3%,高于CK4.7百分点;功率为450W时胚率是49.7%,低于CK10.9百分点。通过方差分析,PSOD=0.0001<0.001,说明超声处理对种子SOD有很高的显著性;PPOD=0.0001<0.001,说明超声对种子POD的影响极显著。结论:可以作为生产上采用超声波处理人参种子的理论依据。

Objective： The Ginseng seed has the character of double dormancy, when the seeds finished morphological after-ripening and physiological after-ripening, it will germinate. Dormancy of seeds had a relationship with It＇s integrity. The enzyme system of seeds is one of important impacts on seed germination physiological parameters. The SOD and POD can impact on seed vigor directly. Methods： In this experiment, ultrasonic method was applied to explore, that how to impact on seed germination. Results： when the temperature is 50 ℃, the rate gap of seed can reach 90 %. When the time is 30 min, the rate gap of seed is 90 %. Double of them are higher than CK is 13 percentage points. When the power is 550 W, the rate gap of seed is 95 %. It is higher than CK 18 percentage points. When the temperature is 30 ℃, the embryo rate of seed is 66. 6 %. When the time is 20 min, the embryo rate of seed is 65.3 %. When the power is 450 W, the embryo rate of seed is 49.7 %. The embryo rate of CK is 60. 6 %. Analysis of variance, PSOD = 0. 000 1 〈 0. 001, so we can see that the ultrasonic method could affect on SOD very extraordinary. And PPOD = 0. 000 1 〈 0. 001, so the affect on SOD is also extraordinary. Conclusion： It could be as the theoretical basis of using ultrasonic on Ginseng seed in practice.