三七总皂苷超声波提取工艺研究

The Process Engineering of Ultrasonic Extraction of Notoginseng Saponins

目的:优化三七中三七总皂苷的超声波提取工艺。方法:采用高效液相色谱法,以提取物中三七总皂苷含量和收率为考察指标,应用正交试验法对提取工艺进行优化。结果:以75%的乙醇为提取溶媒,超声波高频(50kHZ)提取4次,每次30min,提取温度40℃,经纯化后三七总皂苷(以R1、Rg1、Rb1、Re、Rd的总含量计)达80%以上,提取物收率达到12%以上。结论:该工艺简便易行,提取率高,生产成本低,适合工业生产。

Objective： To optimize the technical parameter of ultrasonic extraction of notoginseng saponins （PNS）. Methods： To examine saponin yields by orthogonal design experiments and determine saponin contents by HPLC analysis. Results： An optimize technical parameter of ultrasonic extraction of PNS is high-frequency ultrasonic （50 kHZ）with 75 % ethanol at 40 ℃by extracting 30 minutes every time for four times. PNS content （total content of ginsenosides Rgl, Rbl, Re, Rd and notoginsenoside R, ） is up to 80 % with yield above 12 %. Conclusion： The results show that ultrasonic extract is a suitable method for extraction of PNS and this optimize process could be used in large scale production of PNS.