雷米普利对2型糖尿病大鼠microRNA表达谱的影响

Expression changes of microRNAs in 2 type diabetes' rats treated with ramipril

目的 :建立链脲霉素(streptozocin,STZ)+高糖高脂饮食诱导2型糖尿病大鼠模型,并以血管紧张素转化酶抑制剂(angiotensin-converting enzyme inhibitor,ACEI)药物雷米普利处理,筛查用雷米普利后2型糖尿病大鼠胰腺组织microRNA(miRNA)表达的变化,并进行实时定量PCR验证芯片结果,寻找与2型糖尿病功能改善相关的miRNA。方法:STZ(40 mg/kg)腹腔注射+高脂高糖饲料喂饲健康雄性SD大鼠,测随机血糖≥16.7 mmol/L成模后1周,雷米普利50 mg/(kg.d)灌胃28 d。实验结束后,取胰腺组织常规抽提总RNA,miRNA芯片检测miRNA表达谱的改变。结果:2型糖尿病大鼠经雷米普利处理后,miRNA表达谱中出现明显差异表达(1.5倍以上)的miRNA共有27个,其中20个表达上调,7个表达下调。结论:在2型糖尿病大鼠模型中,用雷米普利处理后部分胰腺miRNA的表达发生明显变化,提示miR-1、miR-30c可能参与胰腺功能改善的调节。

Objective： To investigate the expression pattern of microRNA（miRNA） in pancreas of 2 type diabetes rats treated with ramipril by miRNA array technique.Methods： Male Spmgue-Dawley（SD） rats were injected in peritoneal with streptozocin（40 mg/kg） and fed with high sugar and fat for 1 weeks,then treated with ramipril [50 mg/（kg·d）] for 28 days.When the rats were sacrificed,total RNA was extracted from myocardium and the differential expressions of miRNA were detected by miRNA array.Results： Contrast to control group rats,the expression level of 27 miRNAs changed significantly in ramipril group rats,20 of them was up-regulated and the other 7 were down-regulated.Conclusion： The expression profile of miRNA changes in pancreas of 2 type diabetes rats treated with ramipril,which implies that those changed miRNAs may play an important role in improvement of pancreas function in SD rats.