性激素对女性脂肪组织11βHSD mRNA表达的部位特异性调控

The depot-specific regulation of sex hormones on 11βHSD1 mRNA expression in adipose tissue

目的:观察性激素对育龄期和绝经期妇女不同部位脂肪组织11β羟基类固醇脱氢酶Ⅰ型(11β-hydroxysteroid dehy-drogenase type 1,11βHSD1)mRNA表达水平的调控作用。方法:育龄期非妊娠妇女6例,年龄(29.83±3.43)岁及绝经期妇女16例,年龄(65.19±6.81)岁,取其皮下和网膜脂肪组织进行培养,应用雌激素和雄激素刺激脂肪组织24h,实时定量PCR方法测定脂肪组织11βHSD1 mRNA水平。结果:雌激素可使绝经期妇女皮下脂肪组织11βHSD1 mRNA表达明显增加(P<0.05),使网膜脂肪组织11βHSD1 mRNA表达水平下降30%。雄激素刺激可抑制育龄期妇女皮下脂肪组织11βHSD1 mRNA表达近40%(P=0.001),而使绝经期妇女网膜脂肪组织11βHSD1 mRNA表达增加1.9倍(P=0.005)。结论 :性激素对脂肪组织11βHSD1 mRNA表达的调控呈现年龄和部位特异性改变,可能是不同时期女性体脂分布的重要分子生物学机制。

Objective： To observe the depot-specific regulation of sex hormones on 11β-hydroxysteroid dehydrogenase type 1（11βHSD1） mRNA expression in adipose tissue in child-bearing and menopausal women.Methods： Subcutaneous（Sc） and omental（Om） adipose tissues were obtained from 6 child-bearing（aged 29.83 ± 3.43 years） and 16 menopausal women（aged 65.19 ± 6.81 years）.Pieces of adipose tissue were incubated with estrogen or testosterone for 24h.The expression of 11βHSD1 mRNA in adipose tissues was measured by real time PCR.Results： Estrogen increased 11βHSD1 mRNA expression in Sc adipose tissue（P 0.05） but decreased 11βHSD1 mRNA expression in Om adipose tissue by 30% among menopausal women.Androgen decreased 11βHSD1 mRNA expression in Sc adipose tissue of child-bearing period women by 40%（P = 0.001）,and up-regulated 11βHSD1 mRNA expression in Om adipose tissue of menopausal women by 1.9 fold（P = 0.005）.Conclusion： The sex hormones stimulate 11βHSD1 mRNA expression in adipose tissue in age-and depot-specific pattern,which may be important molecular mechanism of distribution of body fat in child-bearing period and menopausal women.