梗阻性无精子症患者附睾蛋白P34h的表达及其意义

The expression and significance of epididymis protein P34h in obstructive azoospermia patients

目的:探讨梗阻性无精子症患者的附睾组织中附睾蛋白P34h mRNA及蛋白表达情况,并探讨其在梗阻性无精子症患者中的意义。方法:采用逆转录聚合酶链反应(RT-PCR)检测30例因不同梗阻(输精管或射精管水平梗阻)所致的梗阻性无精子症患者附睾组织中P34h基因mRNA的表达水平,Western Blot检测2组P34h蛋白的表达。结果:2组均有P34h mRNA的表达,输精管梗阻的P34h/β-actin均值为0.418±0.045,射精管梗阻的P34h/β-actin均值为0.719±0.069,输精管梗阻的附睾组织中P34h mRNA水平显著低于射精管梗阻(P<0.05)。2组亦均有P34h蛋白的表达,输精管梗阻平均光密度为0.095±0.014,射精管梗阻为0.276±0.054(P<0.05),两组P34h蛋白的表达差异有统计学意义。结论:P34h mRNA及其蛋白在不同梗阻因素所致的无精子症患者的附睾中表达水平有明显差异,提示P34h可能是梗阻性无精子症时导致精子成熟障碍的一个重要因素。

Objective： To explore the expression and significance of P34h in the patients with obstructive azoospermia.Methods：Reverse transcription-polymerse chain reaction（RT-PCR） and Western blot assay were used to examine different mRNA and protein expressions of P34h in epididymis tissues in 30 cases of obstructive azoospermia due to different obstruction sites which located at the vas deferens or ejaculatory duct.Results： The mRNA expression level of P34h in epididymis tissues was different.P34h mRNA expression of the group with obstructive site at vas deferens was lower than that with obstructive site at ejaculatory duct（mean value of P34h/？茁-actin：0.418 ± 0.045 vs.0.719 ± 0.069,P 0.05）.P34h protein in the group with obstruction at vas deferens was significantly lower than that with obstruction at ejaculatory duct（protein：0.095 ± 0.014 vs.0.276 ± 0.054,P 0.05）.Conclusion： The mRNA and protein expressions of P34h were significantly different in epididymis tissues due to various obstructive factors.It may play an important role in sperm mature obstacles in the patient with obstructive azoospermia.