肉鸡胴体中沙门氏菌的分离鉴定及多重耐药谱研究

Separation and identification of multiple-antimicrobial-resistant of Salmonella on chicken carcass

目的:为了解肉鸡胴体中沙门氏菌的污染状况、多重耐药状况及耐药程度,对样品中的沙门氏菌进行分离鉴定和药敏试验研究。方法:根据GB/T 4789—2008对沙门氏菌进行分离鉴定,采用微量肉汤稀释法测定分离株的耐药性。结果:肉鸡胴体沙门氏菌的检出率高达34.8%;样品中分离的沙门氏菌血清型主要为奥尔巴尼型(29.6%)和印第安纳型(50.7%);药敏试验显示,大多数沙门氏菌至少对3种以上的抗生素产生耐药,耐3～7种药物的有24株(33.8%),耐8～12种药物的有19株(26.8%),耐13～16种药物的有26株(36.6%);分离株对抗生素的耐药情况是:萘啶酸(100%)、磺胺嘧啶(98.6%)、氨苄青霉素(97.2%)、复方磺胺甲恶唑(85.9%)、四环素(85.9%)和阿莫西林/克拉维酸(84.5%)。结论:肉鸡胴体中沙门氏菌的污染现象严重,沙门氏菌耐药或多重耐药现象十分普遍,未来应加强对鸡胴体中沙门氏菌感染及耐药的监测。

Objective： To determine prevalence and multiple-antimicrobial-resistant spectrum of Salmonella contamination on chicken carcasses.Methods： According to GB 4789-2008,Salmonellas were separated and identified,and drug resistance of the strains was analyzed using broth dilution method.Results： Salmonella was detected in 34.8%（71/204） of samples.Two Salmonella serovars were identified;predominant ones included Albany（29.6%） and Indiana（50.7%）.Most of the Salmonella isolates tested,exhibited resistance to more than three antimicrobial agents used.Twenty-four strains（33.8%） were resistant to three to seven kinds,nineteen strains（26.8%） were resistant to eight to twelve,and twenty-six strains（36.6%） resistant to thirteen and sixteen antibiotics.Resistance to the following antibiotics was common among isolates：nalidixic acid（100%）,sulphadiazine（98.6%）,ampicillin（97.2%）,sulfamethoxazole（85.9%）,minocycline（85.9%） and amoxicillin/clavulanic acid（84.5%）.Conclusion： High prevalence of Salmonella and the relatively high resistance among the bacteria suggested monitoring of the Salmonella infection and drug resistance on chicken carcass should be strengthened in the future.