摘要
目的:为了解肉鸡胴体中沙门氏菌的污染状况、多重耐药状况及耐药程度,对样品中的沙门氏菌进行分离鉴定和药敏试验研究。方法:根据GB/T 4789—2008对沙门氏菌进行分离鉴定,采用微量肉汤稀释法测定分离株的耐药性。结果:肉鸡胴体沙门氏菌的检出率高达34.8%;样品中分离的沙门氏菌血清型主要为奥尔巴尼型(29.6%)和印第安纳型(50.7%);药敏试验显示,大多数沙门氏菌至少对3种以上的抗生素产生耐药,耐3~7种药物的有24株(33.8%),耐8~12种药物的有19株(26.8%),耐13~16种药物的有26株(36.6%);分离株对抗生素的耐药情况是:萘啶酸(100%)、磺胺嘧啶(98.6%)、氨苄青霉素(97.2%)、复方磺胺甲恶唑(85.9%)、四环素(85.9%)和阿莫西林/克拉维酸(84.5%)。结论:肉鸡胴体中沙门氏菌的污染现象严重,沙门氏菌耐药或多重耐药现象十分普遍,未来应加强对鸡胴体中沙门氏菌感染及耐药的监测。
Objective: To determine prevalence and multiple-antimicrobial-resistant spectrum of Salmonella contamination on chicken carcasses.Methods: According to GB 4789-2008,Salmonellas were separated and identified,and drug resistance of the strains was analyzed using broth dilution method.Results: Salmonella was detected in 34.8%(71/204) of samples.Two Salmonella serovars were identified;predominant ones included Albany(29.6%) and Indiana(50.7%).Most of the Salmonella isolates tested,exhibited resistance to more than three antimicrobial agents used.Twenty-four strains(33.8%) were resistant to three to seven kinds,nineteen strains(26.8%) were resistant to eight to twelve,and twenty-six strains(36.6%) resistant to thirteen and sixteen antibiotics.Resistance to the following antibiotics was common among isolates:nalidixic acid(100%),sulphadiazine(98.6%),ampicillin(97.2%),sulfamethoxazole(85.9%),minocycline(85.9%) and amoxicillin/clavulanic acid(84.5%).Conclusion: High prevalence of Salmonella and the relatively high resistance among the bacteria suggested monitoring of the Salmonella infection and drug resistance on chicken carcass should be strengthened in the future.
出处
《南京医科大学学报(自然科学版)》
CAS
CSCD
北大核心
2012年第1期125-128,共4页
Journal of Nanjing Medical University(Natural Sciences)
基金
卫生部公益性行业科研专项基金(200902009)
关键词
鸡
沙门氏菌属
血清学鉴定
耐药性
chicken carcass
Salmonella
serologic appraisal
drug resistance