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精液标本不同放置时间和温度对其检测结果的影响

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摘要 目的了解精液标本采集后不同放置时间和温度对精子活动率、存活率及功能等检测结果的影响。方法采用西班牙SCA计算机辅助精子质量分析系统检测精子活动率,伊红染色法检测精子存活率,采用精子膜功能测定(低渗膨胀试验)与精子-宫颈粘液相互作用(毛细管穿透试验)测定精子功能。结果精液标本采集后放置60min内检测对精子活动率、存活率及低渗膨胀试验结果无影响(p〉0.05),而放置60min后检测对毛细管穿透试验结果有影响(P〈0.05);其中36℃放置时正常精子组活动率及少精组所有检测结果比25℃更好(P〈0.05),而两种放置温度对弱精组检测结果无影响(P〉0.05)。结论精液标本采集后最好放置在36℃水浴箱中待其液化,液化后宜立刻进行检测,标本采集后1h内读出的检测结果最佳。
出处 《国际医药卫生导报》 2012年第2期247-249,共3页 International Medicine and Health Guidance News
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