地塞米松磷酸钠诱导大鼠阴虚状态的实验研究

Empirical study of yin asthenia state of rats induced by dexamethasone sodium phosphate

目的:观察地塞米松磷酸钠诱导大鼠的病理表现以模拟临床阴虚反应。方法:以0.35mg/kg地塞米松磷酸钠肌肉注射大鼠造阴虚模型,并给模型组大鼠灌胃六味地黄丸溶液,观察和检测相关症状及实验室指标,以分析所造阴虚模型的病理特点。结果:造模期间(第10、21天时),与正常组比较,模型组大鼠的一般症状表现及相关客观生化指标都发生不同程度的改变。实验结束时(造模21d即灌胃11d时),与模型组比较,六味地黄丸组部分异常指标可被调节到正常水平。结论:从与临床病理表现类比及六味地黄丸对其治疗反证,可知地塞米松磷酸钠对大鼠多系统的影响,符合阴虚状态的病理表现,是对临床阴虚状态的模拟再现。

Objective： To imitate yin asthenia reaction in clinic by observing pathological appearance of rats induced by dexamethasone sodium phosphate.Methods： Intramuscular injection with dexamethasone sodium phosphate（0.35mg/kg） of rats to make yin asthenia model which then were intragastricly administrated with solution of six tastes Rehmaniae Bolus.Analysis of the pathological feature of yin asthenia model induced by dexamethasone sodium phosphate by observing and detecting correlated symptoms and laboratory index when experiment completed.Result： During the period of model making（on day 10 and 21）,compared with the normal group,common symptoms and correlated biochemical indicators of rats in the model group all changed at different extent.At the end of the experiment（on day 21）,compared with the model group,some abnormal index can be adjusted to normal of rats in six tastes Rehmaniae Bolus group.Conclusion： From the point of analogy with clinical pathological appearance and disproof by treating with six tastes Rehmaniae Bolus,influences on multisystem of rats induced by dexamethasone sodium phosphate correspond to pathological appearance of yin asthenia state which is the imitation of clinical yin asthenia state.