三七总苷对二甲基亚硝胺致肝纤维化大鼠胶原代谢的影响

Effect of panax notoginsenosides on collagen metabolism of dimethynitrosamine-induced hepatic fibrosis rats

目的:考察三七总苷对二甲基亚硝胺(DMN)致肝纤维化模型大鼠胶原代谢的影响。方法:用DMN复制中度肝纤维化模型,随机分为三七总苷组、干扰素组、三七总苷配合干扰素组、阴性对照组,分别以相应的药物连续治疗8周,检测各组大鼠血清肝功能、透明质酸及肝组织羟脯氨酸(Hyp)含量,结合常规病理HE、苦味酸-天狼星红染色,评价肝组织炎症及纤维化程度。结果:中度肝纤维化大鼠模型经过连续8周的三七总苷治疗,Hyp含量明显少于模型对照组,差异显著(P<0.05),其血清学指标、肝纤维化分级等方面均出现了不同程度的改善。结论:三七总苷能够降低大鼠胶原沉积,减少DMN所致肝纤维化大鼠胶原含量。

Objective： To observe the effect of panax notoginsenosides（PNS） on collagen metabolism of hepatic fibrosis model rats.Methods： DMN hepatic fibrosis model was used in this experimental research.After injected DMN,the rats were divided into 4 groups,including,a.PNS Treatment Group;b.INF-γ Treatment Group;c.PNS INF-γ Treatment Group;d.Negative Control Group;In addition,10 rats were arranged to be the Normal Control Group during the whole study.All rats received 8 weeks＇ treatment by PNS,INF-γ,PNS INF-γ and saline solution,respectively.All rats were killed under anesthesia to observe the effect of PNS on the collagen metabolism of hepatic fibrosis rats compared with INF-γ after 8 weeks＇ treatment.The contents of liver Hydroxyproline,the index of liver function and Hyaluronic Acid（HA） were used to evaluate the function of PNS on the collagen metabolism on hepatic fibrosis rat model compared with IFN-γ.Histological sections of liver from the killed rats were stained by Hematoxylin and Eosin,Sirius Red Stains,respectively.Results： After 8 weeks＇ treatment,the indexes of the liver function and HA in the PNS Treatment Group rats were markedly decreased compared with the Negative Control Group rats（P0.05）,the contents of Hydroxyproline and the grade of hepatic fibrosis in PNS Treatment Group rats liver were lower than those of Negative Control Group,respectively.Conclusion： PNS can improve the collagen contents on hepatic fibrosis rats by not only reduce the index of liver Hydroxyproline,but also by decrease the grade of hepatic fibrosis.