维药洋葱子质量标准研究

Research on quality specification of Allium cepa seed

目的:建立维药洋葱子的质量控制标准。方法:于不同产地采集10批洋葱子样品,对其原植物进行鉴定,对其性状进行鉴别;对其粉末进行显微鉴别;以β-谷甾醇为对照品对洋葱子药材进行薄层色谱鉴别;按照《中国药典》2010年版(一部)附录测定10批洋葱子药材的杂质、水分、总灰分、酸不溶性灰分、过氧化值、浸出物;按照《中国药典》2010年版(一部)附录测定10批洋葱子药材的脂肪油含量。结果:对洋葱子药材的来源、性状进行了描述,对粉末显微进行了鉴别;以环己烷-乙醚-乙酸乙酯(20:5.5:2.5)为展开剂,对洋葱子中β-谷甾醇进行薄层色谱鉴别;检查项中暂定洋葱子药材杂质不得过1.4%,水分不得过10.0%,总灰分不得过12.0%,酸不溶性灰分不得过6.50%,过氧化值不得超过0.13;浸出物不得少于10.00%;根据脂肪油测定结果暂定本品脂肪油含量不得少于9.0%。结论:所制定洋葱子药材的质量控制标准可用于评价洋葱子药材的质量。

Objective： To provide scientific basis for the utilization and development of Allium cepa seed by setting up the quality control specification of Allium cepa seed.Methods： Collecting different origin of 10 batch Allium cepa seed samples,the original plant of which were identificated,the properties of which were described,the powder of which were microscopic identificated.And Allium cepa seed were identificated on TLC method in contrast to β-Sitosterol.Determination of the 10 batch Allium cepa seed of impurities,moisture,ash,peroxide value and extract according to Chinese Pharmacopoeia 2010 version（Vol Ⅰ） of appendix Ⅸ H and K.The fatty oil determination method of Allium cepa seed were inspected according to Chinese Pharmacopoeia 2010 version（VolⅠ） of appendix Ⅸ N.Results： The origins,macroscopic and microscopic identification of Allium cepa seed are described,and the TLC identification method was formulated with thiacyclohexane-ethyl ether-ethyl acetate（20：5.5：2.5） as developing solvent,temporarily formulating impurities can not lead 1.4%,moisture can not lead 10.0%,total ashes can not lead 12.0%,acid-insoluble ashes can not lead 6.50%,peroxide value can not lead 0.13,extract not less 10.00%.Determine the fatty oil of Allium cepa seed shall be not less than 9.0%.Conclusion： Setting the Allium cepa seed quality control standard can be used to evaluate the quality of Allium cepa seed.