Ccbe1在喉癌中的表达及其临床意义

Expression and clinical significance of Ccbe1 in laryngeal cancer

目的探讨胶原和钙结合EGF域1(collagen and calcium binding EGF domains 1,Ccbe1)在喉癌组织中的表达及其临床意义。方法以45例经病理确诊的喉癌组织为实验组,20例声带息肉组织为对照组,采用免疫组织化学和实时荧光定量PCR对上述组织中Ccbe1蛋白表达进行分析,同时采用5'-核苷酸酶染色法(5'-Nase)计数淋巴管密度(lymphatic vessel den-sity,LVD),并结合临床病理特征和生存资料进行相关分析。结果喉癌组织中Ccbe1的蛋白和mRNA表达低于声带息肉组织(P<0.05),在喉癌组织中Ccbe1低表达与肿瘤分化程度、TNM临床分期、淋巴管浸润、淋巴结转移相关(P<0.05),而与年龄、性别、肿瘤部位、LVD不相关(P>0.05)。Ccbe1阳性表达生存率显著高于阴性表达(P<0.05)。结论 Ccbe1是喉癌组织中一种新的肿瘤抑制基因,Ccbe1的检测将有望成为判断喉癌预后的一个新指标。

Objective To investigate the expression of collagen and calcium binding EGF domains 1（Ccbe1）in laryngeal cancer tissues and its relationships with clinical parameters,lymphatic microvessel density and prognosis.Methods The expression of Ccbe1 in 45 cases of laryngeal cancer tissues and 20 cases of polyp of vocal cord tissues was observed and detected by immunohistochemistry and real time fluorescent quantitative PCR.Lymphatic vessel density（LVD）was evaluated by 5′-Nase staining.The correlation of Ccbe1 expression with clinical parameters was analyzed,and the prognosis of laryngeal cancer was analyzed using Kaplan-Meier method.Results The protein and mRNA expression of Ccbe1 was signifi-cantly lower in laryngeal cancer tissue than that in the polyp of vocal cord tissue（P0.05）.The low expression of Ccbe1 in laryngeal cancer tissue was significantly correlated with tumor histological grade,TNM stage,lymphatic metastasis and lymphovascular invasion（P0.05）,but not with age,gender,tumor location and LVD.The survival rate was significantly higher in patients with positive expression of Ccbel than that in negative patients（P0.05）.Conclusion Ccbe1 is a new tumor suppressor gene in laryngeal cancer tissue.The detection of Ccbe1 may be helpful for judging the prognosis of patients with laryngeal cancer.