CK17在宫颈上皮内瘤变和宫颈癌组织中的表达及意义

The Expression and Clinical Significance of Ck17 in Cervical Intraepithelial Neoplasia and Cervical Cancer

目的探讨宫颈上皮内瘤变及宫颈癌中CK17的表达情况及其相互关系及意义。方法应用免疫组织化学S-P法检测15例正常宫颈组织、15例CINⅠ、17例CINⅡ、21例CINⅢ和36例宫颈癌组织中CK17的表达情况。结果 CK17的阳性表达定位于细胞质。在正常宫颈组织、CIN和宫颈癌中,CK17的阳性表达率分别为0、56.6%和83.3%,3组相比,差异有统计学意义(χ2=30.177,P<0.05)。CIN间,CK17阳性表达率分别为20.0%、64.7%和76.2%,CINⅡ、CINⅢ组阳性率均高于CINⅠ组,差异有统计学意义(P=0.016;P=0.002)。随着宫颈病变程度的加重,CK17的阳性表达率逐渐增高,CK17阳性表达率同宫颈病变程度呈正相关关系(γ=0.958,P<0.05)。结论 CK17在宫颈组织恶性转化过程中,随着病变程度的加重,阳性表达率逐渐增高,与病变程度呈正相关。CK17可作为高级别CIN和宫颈癌诊断及预测病变进展趋势的标志物。

Objective To detect the expression and study the significance of CK17 protein in cervical intraepithelial neoplasia（CIN） and cervical cancer samples,and to analyze their correlations.Methods The SP method of immunochemistry was employed to investigate the expression of CK17 in all specimens,including 15 cases of normal cervix epithelia,53 cases of cervical intraepithelial neoplasia and 36 cases of cervical cancer.Results The expression of CK17 was found in cytoplasm.CK17 staining was identified in 56.6% of CIN and 83.3% of cervical cancer cases but was completely absent in reference cervix epithelium.The difference among the three groups was statistically significant（χ2=30.177,P0.05）.In CIN group,its expression was significantly higher in CINⅡ and CINⅢ compared with CINⅠ（P=0.016 and P=0.002 respectively）.Positivity for CK17 correlated with increasing lesion grade of the intraepithelial lesions,the extent of the CK17-positive rate and the grade of the intraepithelial lesions have a positive correlation（γ=0.958,P0.05）.Conclusion The expression of CK17 was significantly correlated with increasing lesion grade of CIN and cervical cancer.It is a valuable marker for monitoring of disease progression in human cervix and the diagnosis of CIN and cervical cancer.