急性白血病患者治疗前后血清IFN-γ、VEGF、IL-10及IL-12水平的变化及意义

Changes and Clinical Significance of Serum Levels of IFN-γ,VEGF,IL-10 and IL-12 Before and After Chemotherapy for Acute Leukemia Patients

目的分析急性白血病(AL)患者化疗前后血清IFN-γ、VEGF、IL-10及IL-12水平的变化。方法采用ELISA双抗体夹心法检测130例急性白血病患者化疗前后IFN-γ、VEGF、IL-10及IL-12水平,并与正常对照组比较。结果化疗前AL患者的VEGF、IL-10、IFN-γ和IL-12活性水平与对照组比较差异有统计学意义(P<0.05)。化疗后AL患者的VEGF、IL-10、IFN-γ和IL-12活性水平与化疗前比较,有统计学意义(P<0.05),其变化情况:长期缓解组>中期缓解组>短期缓解组。结论 IFN-γ、VEGF、IL-10及IL-12参与AL发病,VEGF、IL-10、IFN-γ和IL-12的水平可作为临床上评估AL患者免疫状态和预后的指标。

Objective To study the change of serum levels of IFN-γ,VEGF,IL-10 and IL-12 of acute leukemia（AL）patients before and after chemotherapy.Methods Serum 1evels of IFN-γ,VEGF,IL-10 and IL-12 were detected by ELISA method before and after treatments in 130 patients of acute myeloid leukemia and 50 normal controls.Results The content of IFN-γ,IL-12VEGF and IL-10 were significantly different（P0.05）in acute leukemia patients than in the control subjects at the time of diagnosis.Active level of VEGF,IL-10,IFN-γand IL-12 after chemotherapywere significantly different than before the chemotherapy in AL patients（P0.05）.IFN-γ,VEGF,IL-10 and IL-12 activity level changes after chemotherapy in AL patients：complete remission groupmiddle remission groupshort remission group.Conclusion The level of serum IFN-γ,VEGF,IL-10 and IL-12 are involved in the pathogenesis of AL,the level of which can be used in clinic to assess the immune status of AL patients as well as prognosis indexes.