IL-32及TNF-α在哮喘小鼠肺组织中的表达及布地奈德的干预作用

Expression of Interleukin-32 and Tumor Necrosis Factor Alpha and the effects of Budesonide in mice with asthma

目的:探讨哮喘小鼠发病过程中白细胞介素32(IL-32)及肿瘤坏死因子-α(TNF-α)在肺内表达的变化及布地奈德的干预作用。方法:将30只健康雌性BALB/c小鼠随机分为3组:卵蛋白(OVA)致敏并激发的哮喘模型组(A组),OVA致敏、激发并予布地奈德吸入治疗组(B组)及正常对照组(C组),每组10只。观察卵蛋白激发及布地奈德干预后哮喘小鼠气道的病理变化;免疫组织化学染色观察IL-32及TNF-α表达的变化。结果:IL-32及TNF-α在对照组中呈低表达,在哮喘组中表达明显增加,使用布地奈德干预后,IL-32及TNF-α的表达明显降低(P<0.05),且二者表达量均与气道壁厚度呈正相关(r=0.716,P<0.05;r=0.459,P<0.05)。结论:IL-32及TNF-α可能参与了哮喘小鼠气道重塑过程,布地奈德干预改善哮喘小鼠的气道重塑可能与降低IL-32及TNF-α的表达有关。

Objective：To study the expression of Interleukin-32（IL-32） and Tumor Necrosis Factor Alpha（TNF-α） in the airway and the effect of Budesonide on their expression in mice with asthma.Methods：Thirty BALB/c Female mices were randomly divided into three groups： placebo control group,untreated asthma group,and Budesonide-treated asthma group.The asthma group were induced by intraperitoneal injection of 10% ovalbumin（OVA） on days 1,8 and 15,and then from days 22 to 34,challenged by inhalation of 2% OVA aerosol every other day.The Budesonide-treated asthma group received an inhalation of Budesonide（1 mg） before OVA challenge.The pathological changes of the airway were assessed by hematoxylin and eosin staining.The immunohistochemistry was used to estimate the expression of IL-32 and TNF-αin the lung.Results：The expression of IL-32 and TNF-αin the lung was low in the control group and increased significantly in the untreated asthma group（P0.05）,but decreased significantly in the Budesonide-treated asthma group..Both IL-32（r=0.716,P0.05） and TNF-α（r=0.459,P0.05） were positively correlated with the thickness of the airway wall.Conclusion：IL-32 and TNF-α may be associated with airway remodeling inmice with asthma.Budesonide can improve airway remodeling,possibly by decreasing the expression of IL-32 and TNF-α.