盐碱胁迫对沙棘种子萌发及幼苗抗氧化酶活性的影响

Effects of Salt and Alkali Stresses on Germination and Antioxidant Enzyme Activities of Hippophae rhamnoides Seedlings

采用不同浓度NaCl和NaHCO3溶液处理沙棘(Hippophae rhamnoides L.)种子,并测定其种子萌发率、幼苗鲜质量和抗氧化酶活性。研究结果表明:10～30 mmol.L-1NaCl溶液处理沙棘种子,种子能萌发;40～100mmol.L-1 NaCl和10～20 mmol.L-1 NaHCO3溶液处理的种子萌发受到明显抑制,125～200 mmol.L-1 NaCl和30～200 mmol.L-1 NaHCO3溶液处理的种子完全不萌发。随着NaCl(10～100 mmol.L-1)和NaHCO3(10～20 mmol.L-1)胁迫强度增加,沙棘幼苗鲜质量表现为下降的趋势,SOD、CAT活性呈先增加后降低趋势,POD活性基本呈增加趋势。

An experiment was conducted to study the seed germination rate,fresh weight and activities of antioxidant enzymes of Hippophae rhamnoides seedlings treated with different concentrations of NaC1 or NaHCO3.Results showed that the seeds treated with 10-30 mmol·L-1 NaC1 could normally germinate,and the seeds would be obviously inhibited under the condition of 40-100 mmol·L-1 NaC1 or 10-20 mmol·L-1 NaHCO3.However,the seeds did not germinate under 125-200 mmol·L-1 NaC1 or 30-200 mmol·L-1 NaHCO3.With increasing concentration of NaCl（10-100 mmol·L-1） or NaHCO3（10-20 mmol·L-1）,the fresh weight of H.rhamnoides seedlings declined,while activities of superoxide dismutase（SOD） and catalase（CAT） first increased and then decreased,and peroxidase（POD） activity increased.