一株高效溶磷小麦内生菌的筛选与鉴定

Screening and Identification of a Phosphate-Dissolving Endophyte from Wheat

从小麦根、茎、叶分离出21株溶磷微生物,筛选出溶磷能力最强的菌株JG-22,经形态特征、生理生化分析及16S rDNA序列分析,确定其为土壤芽孢杆菌(Brevibacillus agri strain).研究表明,JG-22对Ca3(PO4)2溶解能力最强;pH值对菌株JG-22溶磷效果影响最明显,在34～36℃和弱碱性环境下,溶磷效果较好;培养72 h后增加培养时间对有效磷的增量影响不大;Ca3(PO4)2含量在5 g/L以上时加大其含量对有效磷的增量几乎无影响.

21 strains of phosphate-dissolving bacteria were separated from wheat roots,stems and leaves and the most powerful phosphate-dissolving was strain JG-22.The characteristics of morphology,physiological and biochemical analysis and 16S rDNA series analysis showed that JG-22 belonged to Brevibacillus agri strain.The study indicated the ability of dissolving Ca3（PO4）2 of JG-22 is the strongest.In 34~36 ℃ and weak alkaline environment,the effect of phosphate-dissolving of JG-22 is evident.