M_2受体抗体在肺源性心脏病发病中大鼠心脏结构影响的实验研究

Effects of muscarinic receptor M2 antibody on heart structure in rats with cor pulmonale

目的 通过建立肺源性心脏病大鼠模型观察M2受体抗体对心脏结构的影响。方法 60只Wistar大鼠随机分为5组(每组12只):单纯缺氧组、环孢素A组、尼卡地平组、对照组、主动免疫组,测定各组大鼠血清中M2受体抗体的含量,光镜及电镜下观察各组大鼠心脏结构改变并计算相关指标。结果 M2受体抗体在单纯缺氧组为67%,主动免疫组为100%,与对照组(阳性率为8%)比较差异均有统计学意义(P<0.01)。环孢素A组与对照组比较差异无统计学意义(P>0.05);与对照组比较,单纯缺氧组及主动免疫组心脏结构有明显变化,环孢素A组与对照组比较差异无统计学意义(P>0.05)。结论 M2受体抗体在引起心脏结构病理改变的过程中具有重要作用。

Objective To investigate the effects of muscarinic receptor M2 antibody on the heart structural in established rat models of cor pulmonale. Methods A total of 60 rats were randomly allocated into 5 groups （n=12 each）： hypoxia group, cyclosporine A group, nicardipine group, control group and active immunized group, respectively. In these groups, the level of serum muscarinic receptor M2 antibody was measured, and the changes in heart structure were observed with light and electronic microscopes for computation of relevant parameters. Results The positive rate of muscarinic receptor M2 antibody was 67% and 100%, respectively, in hypoxia and active immunized groups, with statistical difference （P0.01） as compared with control group （8%）. This value did not differ significantly between cyclosporine A group and control group （P0.05）. There was a remarkable alteration in heart structure in both hypoxia and active immunized groups as compared control group. No structural difference was revealed in cyclosporine A group as compared with control group （P0.05）. Conclusion Muscarinic receptor M2 antibody plays a pivotal role in the pathological change of heart structure.