摘要
采用热水浴法提取山楂多糖,正交设计优化提取条件,并将山楂多糖用于保加利亚乳杆菌的增殖试验。结果表明,山楂多糖的最佳提取条件为:料液比1∶20、提取时间6h、提取温度90℃。在此条件下,山楂多糖的提取率为3.92%。山楂多糖对保加利亚乳杆菌有促生作用并且在多糖添加量为2%,pH值为6.5,温度为42℃时促进效果最好。通过绘制生长曲线与普通培养基中生长曲线相比较,证明山楂多糖对保加利亚乳杆菌的生长有明显的促进作用。
The Hawthorn polysaccharide was extraction with the method of hot bath,and its effect on the growth of Lactobacillus bulgaricus was evaluated.The extraction conditions were firstly optimized by the orthogonal experiment design.The results showed that the optimal extraction conditions were as follows： solid-liquid ratio 1∶ 20,extraction time 6h and extraction temperature 90℃.Under these conditions,the polysaccharide extraction ratio was up to 3.92%.The hawthorn polysaccharide had a great growth-promoting effect on L.bulgaricus when polysaccharide 2%,pH value 6.5 and temperature 42℃.By comparing the growth curves,it showed that hawthorn polysaccharide had obvious positive effect on the growth of L.bulgaricus.
出处
《中国酿造》
CAS
2012年第1期136-140,共5页
China Brewing
关键词
山楂多糖
提取
保加利亚乳杆菌
促生作用
hawthorn polysaccharide
extraction
Lactobacillus bulgaricus
growth-promoting effect
