粒细胞巨噬细胞集落刺激因子与乙肝疫苗协同诱导小鼠细胞免疫应答

Cellular immune responses induced by hepatitis B vaccine associated with Granulocyte-macrophage colony stimulating factor

目的考察粒细胞巨噬细胞集落刺激因子(Gm-csf)对乙型肝炎疫苗免疫原性及诱导的细胞免疫效果的加强作用。方法将Gm-csf和乙型肝炎疫苗分别采用4种不同方式免疫BALB/c小鼠,免疫后,制备脾细胞,再以相应的HBsAg体外刺激,酶联免疫法(ELISA)测定细胞因子分泌水平,MTT法测定脾细胞对抗原刺激指数;以YAC-1细胞为靶细胞,测定细胞毒性T淋巴细胞活性;同时ELISA法测定血清中特异性抗体IgG1和IgG2a。结果GM+疫苗组小鼠脾细胞产生的IFN-γ水平最高,显著高于其它组(P<0.05),而各免疫组IL-5水平没有显著差别(P>0.05);GM+疫苗组小鼠脾细胞对抗原刺激的刺激指数显著高于其它各组(P<0.05);GM+疫苗组小鼠脾细胞对YCA-1的杀伤性显著高于其它各组(P<0.01)。结论通过Gm-csf的联合免疫,可提高乙型肝炎疫苗的免疫原性,增强乙型肝炎疫苗诱导的细胞免疫水平。

Purpose To investigate the enhanced cellular immunity responses induced by Granulo- eyte-macrophage colony stimulating factor（Gm-csf） cooperated with hepatitis B vaccine in BALB/C mice. Methods All BALB/C mice were immunized with hepatitis B vaccine and Gm-csf in four different ways, respectively. Splenic lymphocytes of mice were prepared after immunization. The cytokines concentration of IFN-~/and IL-5 were detected by ELISA and the stimulation indexes were detected by MTT after stimu- lation with HBsAg in vitro. CTL activity was tested in against YAC-1. Then we examined the specific an- tibAies of sera（IgG1 and IgG2a）. Results The concentration of IFNsecreted by mice immunized with ＂ GM ＋ vaccine＂ group was significantly higher than that of other groups （P 〈 0.05 ）. And there was no significant difference of the IL-5 level（P 〉 0.05 ）. The stimulation index of ＂ GM ＋ vaccine＂ group was significantly higher than that of other groups （ P 〈 0.05 ）. The specific cytolytic activity of splenocyte of mice immunized with ＂ GM ＋ vaccine＂ group was the highest（P 〈 0.01 ）. Conclusion The immunogenici- ty of hepatitis B vaccine and cellular immunity responses induced by hepatitis B Vaccine could be im- proved by Gm-csf.