浸麻芽孢杆菌环糊精葡糖基转移酶的克隆与表达

Clone and expression of cyclodextrin glycosyltransferase from Bacillus macerans in Escherichia coli

目的获取浸麻芽孢杆菌环糊精葡糖基转移酶基因并在大肠杆菌中表达。方法构建浸麻芽孢杆菌环糊精葡糖基转移酶表达质粒,转化大肠杆菌DH5α,筛选获得阳性重组菌株。经42℃诱导后,检测酶活性。结果浸麻芽孢杆菌环糊精葡糖基转移酶在大肠杆菌中成功表达,表达量达3 U/mL。结论浸麻芽孢杆菌环糊精葡糖基转移酶可以在大肠杆菌中高效表达。

Purpose To obtain the gene of cyclodextrin glycosyltransferase from Bacillus macerans and to express it in Escherichia coll. Methods The expression plasmid of cyclodextrin glycosyhransferase from Bacillus macerans was constructed ,then it was transferred into Escherichia coli DH5x, and positive clones were obtained by screening. After induction, the enzyme activity was detected. Results The cyclodextrin glycosyltransferase from Bacillus macerans was successfully expressed in Escherichia coli,and the enzyme activity was 3 U/mL. Conclusion The cyclodextrin giycosyltransferase from Bacillus macerans can be ex- pressed efficiently in Escherichia coll.