摘要
采用同位素稀释法结合固相萃取净化,建立了海产品中苯并(a)芘残留的气相色谱串联质谱(GC-MS/MS)检测方法。样品经乙腈-丙酮(体积比6∶4)溶液提取,硅胶固相萃取净化,苯并(a)芘用GC-MS/MS测定,同位素D12-苯并芘内标法定量。方法的平均回收率为93%～98%,相对标准偏差为6.2%～12.2%(n=6),定量限为0.2μg/kg。用该方法测定FAPAS烟熏鱼有证标准样品,测定值与标准值一致。
An analytical method for the determination of Benzo（α）pyrene in seafood was developed by isotope dilution gas chromatography tandem mass spectrometry（GC-MS / MS）. Sample was extracted by using acetonitrileacetone mixture as solvent, purged by silica solid phase extraction column and analyzed by GC-MS/MS under selective reaction monitoring mode. Three deuterated surrogate standards Benzo（a）pyrene-D12 were used as internal standards for quantification and analytical quality control. The limit of quantification（LOQs） was 0.2μ g/kg,the recoveries ranged from 93% to 98%, and the relative standard deviations were 6.2%-12.2%（n=6）. The method was used to determine the quality control materials T0642 provided by the FAPAS and the results were consistent with standard values.
出处
《化学分析计量》
CAS
2012年第1期24-26,共3页
Chemical Analysis And Meterage
基金
国家质检总局科技计划项目(2011IK222)
