Kiss-1在大肠癌中表达的研究及其与NF—κB的关系

Expression of Kiss - 1 protein in colorectal carcinoma and its correlation with expression of NF - κB

目的探讨Kiss-1与NF—κB在大肠癌中的表达及其与生物学行为的关系，并分析两者的关系。方法应用免疫组化sP法检测Kiss-1、NF—κB在103例大肠癌及正常大肠黏膜组织中的表达。结果（1）Kiss-1蛋白在大肠癌中的阳性率为43．69％（45／103），在正常组织中的阳性率为79．61％（82／103），两者问的差异有统计学意义（P〈0．05），Kiss-1在癌组织的表达明显低于对照组；NF—κB蛋白在大肠癌中的阳性率为66．02％（68／103），在正常组织中的阳性率为22．33％（23／103），两者间的差异有统计学意义（P〈0．05），NF—κB在癌组织的表达明显高于对照组。（2）Kiss-1在大肠癌中的表达与淋巴结转移及临床分期有关（P〈0．05）。（3）NF—κB在大肠癌中的表达与淋巴结转移、临床分期及浸润深度有关（P〈0．05），Kiss-1及NF—κB与性别、年龄等其他临床病理参数无关。（4）Kiss-1与NF—κB在大肠癌中的表达呈负相关（X2=6．7811，P〈0．05）。结论Kiss-1和NF—κB的异常表达可能与大肠癌的发生发展密切相关，两者在肿瘤的恶性进程中扮演重要角色。

Objective To study the relationship between the expression of Kiss - 1 and its bio- logical behavior, as well as its correlation with the expression of NF - κB in colorectal carcinoma. Methods The expression of Kiss - 1 and NF -κB were detected by immunohistochemical staining in 103 cases of colorectal carcinoma tissues,and were compared with those in 103 cases of normal eoloreetal tissues. Results （ 1 ） The positive rate of Kiss - 1 protein was 43.69% （45/103） in colorectal carcinoma tissues and 79.61% （82/103）in normal colorectal tissues;There was significant difference between the two groups. The positive rate of Kiss - 1 protein was lower in tumor than that in normal colorectal tissues. The positive rate of NF - κB protein was 66.02% （68/103） in colorectal carcinoma tissues and 22.33% （23/103） in normal colorectal tissues; There was significant difference between the two groups. The positive rate of NF - κB protein was higher in tumor than that in normal colorectal tissues. （2）The expression of Kiss - 1 in colorectal carcinoma tissues correlated with the metastasis of lymph nodes and the clinical stage （ P 〈 O. 05）. （3）The expression of NF -κB in colorectal carcinoma tissues correlated with the metastasis of lymph nodes, the clinical stage, and the depth of invasion（ P 〈 0.01 ）. The expression of Kiss - 1 and NF - KB in colorectal carcinoma did not significantly correlate with the sex or age of patients and so on. （4）The expression of Kiss - 1 negatively correlated with NF - κB （ X2 = 6.7811 ,P 〈 0.01 ）. Conclusion The ab- normal expression of Kiss - 1 and NF -κB are important genetic events associated with the genesis and development of colorectal carcinoma.