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丁酸钠对人结肠癌细胞株HCT-116细胞凋亡及基质相互作用分子和Orai1蛋白活性的影响 被引量：5

Effect of sodium butyrate on apoptosis and stromal interaction molecule and Orail activity in human colon cancer HCT-116 cells in vitro
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摘要 目的研究丁酸钠诱导结肠癌细胞株HCT-116细胞凋亡机制。方法 hoechst 33342和AnnexinV+PI检测丁酸钠诱导结肠癌细胞HCT-116的凋亡作用;免疫荧光法检测丁酸钠对基质相互作用分子(STIMl)和钙离子通道Orail蛋白在细胞内定位的影响;免疫印迹法检测STIMl和Orail蛋白表达量的变化。结果丁酸钠可诱导结肠癌细胞HCT-116凋亡、STIMl移位并与Orail具有共定位现象。结论丁酸钠可通过调节STIM1和Orail在结肠癌细胞中的定位而促发细胞凋亡。 Objective To investigate the mechanism underlying sodium butyrate（NaB）-induced apoptosis of a human colon cancer cell line HCT-116.Methods The apoptosis of HCT-116 cells induced by NaB was confirmed by hoechst33342 staining and AnnexinV＋ PI assay.The changes in the intracellular localization of stromal interaction molecule（STTM1） and Orai1 following NaB treatment were detected by immunofluorescence technique.Western blotting was used to investigate the protein expression levels of STIM1 and Orai1.Results NaB induced apoptosis and caused translocation and colocalization of STIM1 and Orai1 in HCT-116 cells.Conclusion The apoptosis of human colon cancer cells induced by NaB is correlated to the redistribution of STIM1 and Orai1.
作者 孙素霞 李文军 陈思强 张贺 余少珍 张敏红 邹飞
机构地区 南方医科大学公共卫生与热带医学学院营养与食品卫生学系 南方医科大学公共卫生与热带医学学院职业卫生与职业医学系 南方医科大学公共卫生与热带医学学院佛山出入境检验检疫局
出处 《南方医科大学学报》 CAS CSCD 北大核心 2012年第2期189-192,共4页 Journal of Southern Medical University
基金 国家自然科学基金(30971193)~~
关键词 丁酸钠 人结肠癌细胞 细胞凋亡 STIM1 Orai1 sodium butyrate human colon cancer cells apoptosis stromal interaction molecule Orai1
分类号 R735.35 [医药卫生—肿瘤]
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参考文献12

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同被引文献76

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南方医科大学学报
2012年 第2期

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