顺式CA1P对肿瘤细胞增殖和血管生成的影响

Cis-CA1P inhibits tumor cell proliferation and prevents blood vessel formation

目的:研究顺式考布他汀二磷酸四钠(cis-CA1P)对体外培养肿瘤细胞增殖的作用,以及对鸡胚尿囊膜血管和离体培养的大鼠主动脉环血管生成的影响。方法:通过测定MTT评价药物对体外培养肿瘤细胞的作用;建立培养鸡胚尿囊膜模型、离体培养大鼠动脉环,研究顺式CA1P对血管生成的影响。结果:顺式CA1P对MGC-803人胃癌细胞、U937人急性髓系白血病细胞、A375人黑色素瘤细胞、HCT116人结肠癌细胞、MDA-MB-231人乳腺癌细胞、K562人白血病细胞具有显著的生长抑制作用,且呈明显的浓度依赖性;顺式CA1P呈剂量依赖性抑制鸡胚尿囊膜血管及离体培养的大鼠主动脉环血管生成,并可以抑制血管内皮细胞的运动及血管网络状结构形成。结论:顺式CA1P具有抑制肿瘤细胞增殖和抗血管生成的作用。

Objective： To investigate the effects of ciscombretastatinA1 phosphate（cis-CA1P） on tumor cell proliferation,and its effects on the blood vessel formations.Methods： MTT and IC50 values were used to assess the inhibitory effects of cisCA1P on tumor cell proliferation.Chicken embryo chorioallantoic membrane and thoracic aorta annulations isolated from rats were used to investigate the effects of cis-CAIP on the blood vessel formation.Results： Cis-CA1P concentration-dependently inhibited the proliferations of several cancer cell lines,including human gastric carcinoma cell line MGC-803,human leukemic monocyte lymphoma cell line U937,human melanoma cell line A375,human colon cancer cell line HCT116,human breast carcinoma cell line MDA-MB-231,and human leukemia cell line K562.Cis-CAIP signi-ficantly decreased the formation of blood vessels in chicken embryo chorioallantoic membrane and in thoracic aorta annulations.Conclusion： Cis-CA1P inhibits cancer cell proliferation and prevents blood vessel formation.