厄贝沙坦对人脐静脉内皮细胞株EA.hy926增殖、凋亡、VEGF mRNA表达的影响

Effect of irbesartan on the proliferation,apoptosis and VEGF mRNA expression of human umbilical vein cell line EA.hy926 in vitro

目的:应用不同浓度厄贝沙坦对人脐静脉内皮细胞株EA.hy 926的增殖、凋亡生物学效应及血管发生主要基因VEGFmRNA的表达进行体外研究,探讨厄贝沙坦对内皮细胞的血管生成效应。方法:各种浓度厄贝沙坦对人脐静脉内皮细胞株EA.hy926共同孵育24 h。细胞增殖采用CCK8法分析,Annexin V/PI双染法检测细胞凋亡。RT-PCR验证VEGFmRNA的表达。结果:厄贝沙坦各浓度干预组细胞形态无明显变化,CCK8结果提示厄贝沙坦各干预组相比对照组细胞增殖活力增高(P<0.05),呈浓度非依赖性。流式细胞仪分析厄贝沙坦各浓度干预组细胞无明显凋亡。RT-PCR发现厄贝沙坦1×10-4,1×10-5,1×10-6mol/L浓度组VEGFmRNA表达增高(P<0.05)。结论:厄贝沙坦促进EA.hy926细胞株细胞增殖,上调VEGFmRNA的表达。这提示除了降压效应,血管紧张素受体拮抗剂在缺血性心脏病如慢性心力衰竭治疗中具有一定作用。

Objective： To evaluate the effect of irbesartan on the proliferation,apoptosis,and VEGF mRNA expression of human umbilical vein cell line EA.hy926 in vitro.Methods： The human umbilical vein cell line EA.hyY926 were treated with various concentrations of irbesartan for 24 h.The cell proliferation after the treatment was detected by CCK8 assay,flow cytometry and FITC Annexin V/PI kit were used to detect changes in the cell apoptosis.RT-PCR was used to evaluate the expression of VEGF mRNA.Results： There were no changes in cell shape with various concentration of irbesartan.CCK-8 assay showed a greater rate of the cell proliferation in irbesartan group than that in control group with a dose-independent manner after 24 h treatment.After incubation with irbesartan,cell proliferation rate was significant（P0.05）.FCM analysis showed no significantly changes in the cell apoptosis.Irbesartan increased the proliferation of EA.hy926 cells.At concentration of 1×10-4,1×10-5,1×10-6 mol/L,VEGF mRNA expression enhanced either（P0.05）.Conclusion： Irbesartan could promote the proliferation and up-regulated VEGFmRNA expression in EA.hy926 cell line.This result suggested that in addition to antihypertensive effect,angiotensin receptor antagonist might be a novel therapeutic approach to chronic ischemic heart disease as heart failure.