利用同步辐射X线成像技术进行大鼠正常真皮及瘢痕真皮组织的三维结构研究

Exploring the three-dimensional structure of dermal tissues of normal skin and scar in rat with syn- chrotron radiation X-ray imaging technology

目的 观察比较大鼠正常真皮与瘢痕真皮组织形态学差异并探寻其结构性规律.方法 切取SD大鼠全层皮肤和创面愈合后3周的瘢痕组织,制备样本进行适当处理,结合同步光源辐射技术、显微CT及相位衬度成像技术进行断层成像,用三维软件进行图像重建. 结果 利用同步辐射光源产生的X射线,使显微CT成为可能；在处理过程中结合相位成像技术,能够清晰地呈现真皮组织结构.正常真皮组织以众多胶原纤维为主,相互盘绕形成橄榄状,并以此为基本单元,按某种形式错落组成.瘢痕真皮组织胶原纤维呈平行排列,部分纤维弯曲,排列混乱. 结论 正常大鼠真皮组织具有稳定的三维结构,其基本框架和组合规律与瘢痕真皮之间存在明显差异.

Objective To compare the morphological difference between dermal tissue of normal skin and that of scar in rat,and to explore its structural pattern. Methods The full-thickness skin and the scar tissue formed 3 weeks after wound healing from SD rats were harvested as samples,which were prepared appropriately afterwards.Samples were scanned and imaged with synchrotron radiation technology,micro-CT,and phase-contrast imaging technology.The images were rebuilt with three-dimensional software.Results The micro-CT was materialized by using X-ray generated by synchrotron radiation light source.The structure of dermal tissues was clearly shown with the assistance of phase-contrast imaging technology in the process.It was demonstrated that the dermal tissues of normal skin of rat were mainly composed of collagenous fibers,which twined together to form an olive-like structure.These olive-like structures as basic units were arranged randomly in a certain way.The collagenous fibers in dermal tissue of the scar were arranged in a parallel manner,while some fibers were crooked and arranged in a disorderly manner. Conclusions Dermal tissue of normal skin in rat has stable three-dimensional structure,and its basic structure and manner of composition are obviously different from those of sear dermal tissue.