155株胆道感染病原菌的分布及耐药性调查

Distribution and antimicrobial resistance of 155 strains of pathogens causing infection of biliary tract

目的了解医院胆道感染病原菌的分布及耐药性,为临床合理使用抗菌药物提供试验依据。方法胆汁培养与菌种鉴定按照《全国临床检验操作规程》进行;药敏试验按K-B法进行。结果胆汁标本细菌阳性分离率为60.3%,其中,革兰阴性杆菌占69.0%;革兰阳性球菌占31.0%;药敏试验表明,除肠杆菌科细菌对碳青霉烯类抗菌药物100.0%敏感、革兰阳性球菌对糖肽类抗菌药物100.0%敏感以外,155株病原菌对其他常用抗菌药物显示了较为严重的耐药性。结论胆道感染病原菌的耐药性日趋严重,临床应按照药敏结果针对性用药。

OBJECTIVE To understand the distribution and antimicrobial resistance of pathogens causing biliary tract infection and provide the experimental evidence for clinical use of antibiotics. METHODS Referring to National Guide to Clinical Laboratory Operation Procedures,bile culture and strain identification of bacteria were performed.Susceptibility testing was performed by KB methods. RESULTS Positive isolating rate was 60.3%（155/257）.The gram-negative bacilli and gram-positive coccus accounted for 69.0% and 31.0%,respectively.Susceptibility testing results indicated that except for 100.0% susceptibility to carbapenems against Enterobacteriaceae and glycopeptide antibiotics against gram-positive coccus,155 strains of pathogens had been highly resistant to the other commonly used antibiotics. CONCLUSION The antimicrobial resistance of pathogens causing biliary tract infection is worsening.Clinicians should use antibiotics according to the drug susceptibility results.