临床标本中假丝酵母菌属的检出率及耐药性分析

Detection rates and drug resistance of Candida from clinical specimens

目的了解医院假丝酵母菌属感染的临床分布及耐药率。方法以萨布罗培养基分离真菌,用API 20CAUX假丝酵母菌属鉴定板和ATB 3Fungus假丝酵母菌属药敏板进行假丝酵母菌属的鉴定和药敏检测。结果共分离假丝酵母菌属189株,检出率为9.6%,以白色假丝酵母菌的检出率居首位,占76.2%,其次为热带假丝酵母菌、光滑假丝酵母菌、近平滑假丝酵母菌,分别占8.5%、6.3%、5.3%;189株假丝酵母菌属所致的临床感染,主要为肺部感染,痰标本中共检出114株,占60.3%,其次为清洁中段尿、粪便,分别占15.3%、9.0%;189株假丝酵母菌属对5-氟胞嘧啶、两性霉素B、伏立康唑的敏感率较高。结论必须重视假丝酵母菌属的培养鉴定和药敏试验,以指导临床合理使用抗真菌药物。

OBJECTIVE To analyze the distribution and drug resistance of clinical Candida. METHODS Sobaurand＇s agar culture medium was used for separating fungi.Fungi were identified by API20C AUX strips and susceptibility was detected with the ATB3 FUNGUS strips. RESULTS 189 strains of Candida strains were identified.Among all strains of Candida,the majority were C.albicans（76.2%）,followed by C.tropicalis（8.5%）,C.glabrata（6.3%）,C.parapsilosis（5.3%）.189 strains were isolated from sputum,urine,stool,blood,accounting for 60.3%,15.3% and 9.0%,respectively.The susceptibility of 189 strains of Candida to 5-fluorocystine,amphotricin B,voriconazole were low. CONCLUSION It is important to perform strain identification and susceptibility of Candida species to guide the rational clinical use of antifungal drugs.