摘要
以纳米蒙脱石(MNC)为吸附材料,重金属铅为吸附对象,以"杜长大"三元杂交猪为试验动物,研究了MNC驱铅对生长肥育猪机体免疫与抗氧化性能的影响。免疫学指标测定结果表明,与10mg/kg铅组比较,10mg/kg铅+0.5%MNC组猪的胸腺、脾脏系数分别减少了27.97%(P<0.01)、12.37%(P<0.05),T-淋巴细胞转化力、血清IgG、IgM水平分别提高了17.89%(P<0.05)、16.20%(P<0.05)、14.46%(P<0.05)。试验各组的IgA水平均未发生显著性变化(P>0.05)。抗氧化能力研究表明,与对照组相比,加MNC组猪血清与肝脏中MDA含量显著降低(P<0.05),CTA、SOD、GSH-Px活力均显著提高(P<0.05);与10mg/kg铅组比较,添加10mg/kg铅+0.5%MNC组猪血清和肝脏中MDA含量明显下降(P<0.05),CTA、SOD、GSH-Px活力明显上升(P<0.05)。此外,饲料中添加MNC后显著提高了猪肝脏中MT、K+,Na+-ATPase和Ca2+,Mg2+-ATPase酶活(P<0.05)。结果表明,MNC能有效吸附饲料中的铅,阻断铅经消化道进入动物体内,改善猪的免疫与抗氧化性能。
Ninety-six pigs(Duroc×Landrace×Yorkshire) were randomly divided into four groups,each of which was replicated three times with eight pigs.The groups received the same basal diet supplemented with 0,0.5%MNC,10 mg/kg Pb and 10 mg/kg Pb+0.5%MNC,respectively.Results showed Pb increased lipid peroxides level,thymus and spleen index,reduced ConA stimulation index of lymphocyte,IgG and IgM in serum and liver,along with a concomitant decrease in the activities of superoxide dismutase,catalase and glutathione peroxidase in liver,but in reverse while MNC was added into the diet.Moreover,the activities of hepatic MT,K+,Na+-ATPase and Ca2+,Mg2+-ATPase were also significantly reduced when fed diets supplemented with MNC.These results indicated that MNC is possible to improve the immunity and antioxidant capacity of pigs as a potential sorbent in Pb-contaminated feed.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2012年第2期285-289,共5页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(021122680)